| Background and purpose:Ethanol is an ester dissolving solvent, and it can enter the brain through the blood. And it produced the toxic function directly in brain nerve cell. Ethanol can cause the damages of nerve system. With the rising of ethanol density in the blood, people’s attention, judgement and discrimination will be decreased in various degree, and may initiate various criminal cases and civil cases. In recent years, with more and more ethanol drinks appeared in people’s living, various civil and criminal cases and traffic accident are on the rise. So it is very necessary to carry on deep research to the mechanisms of ethanol and organism’s function. In this research, we measure the changes of extracellular regulate protein kinase (extracellular regulated protein kinases ERK) phosphorylation and MAPKs signal in the rats’brain nerve cell after poisoned by ethanol, and detect its relationship of morphology changes, finally offer the reference basis for the injury function of the nervous system by ethanol.Method:1. According to the raitio of4ml/k、8ml/kg、16ml/kg prefused to rates respectivelytu produce low, medium and high dose of acute aclohollism model,the corresponding control groupwas given the correspond volume saline.2. Using the immunohistochemistry technique to detect p-ERK1/2expreession in the cerebral nerves cell of rats with different concentrations of ethanol.3. Using the Western-blot technique to detect p-ERK1/2expreession in the cerebral cortex of rats with different concentrations of ethanol.4. Statistical analysis:all the data were dealt with SPSS10.0statistical software package and the measurement data were indicated with mean±standard deviation (x±s). Comparing groups used ANOVA, LSD test and a value of P<0.05was considered as statistical significant. Western-blot data were dealt with by the mageJ image processing system.to The determination of the target article With a gray value.Result:1. The results of H.E. Staining:The low group and control group at different time have not been obviously pathological changes in brain tissue:There were more obvious laeunae widen of nerve cells and small blood vessels in medium-dose ethanol group changed after4h and8h; There were most obviously laeunae widen of nerve cells and small blood vessels in High-dose group changed after2h,4h and8h.2. The results of rats’brain immunohistochemistry indicated that there were significant changes in somatosensory central, auditory center, hippocampus, mediorsal nucleus, hypothalamus and amygdale. The intensity of p-ERK1/2in cerebral cortex and hippocampus of the control group was less expressed:The expression of p-ERK1/2in cerebral cortex and hippocampus of the low-dose ethanol group was increased; there were statistical significance in cerebral cortex of the low-dose ethanol group at1h,2h and4h compared to control group(P<0.05);there were statistical significance in hippocampus of the low-dose ethanol group at2h and4h compared to control group(P<0.05).There were statistical significance in cerebral cortex of the medium-dose ethanol group at1h,2h,4h and8h compared to control group(P<0.05);there were statistical significance in hippocampus of the medium-dose ethanol group at2hcompared to low group(P<0.05); there were statistical significance in cerebral cortex and hippocampus of the high-dose ethanol group at1h,2h,4h,8h and12h compared to control group(P<0.05);there were statistical significance in cerebral cortex and hippocampus of the high-dose ethanol group at1h,2h,4h and8hcompared to control group(P<0.05);there were statistical significance in cerebral cortex and hippocampus of the medium-dose ethanol group at lh,2h and4h compared to medium group(P<0.05). The intensity of p-ERK1/2in mediorsal nucleus of the control group was higher than cerebral cortex and hippocampus.The expression of p-ERK1/2in mediorsal nucleu of the low-dose ethanol group was increased, there were statistical significance of the low-dose ethanol group at2h compared to control group(P<0.05):There were statistical significance of the medium-dose ethanol group at1h,2h and4h compared to control group(P<0.05);there were statistical significance of the medium-dose ethanol group at2h compared to low group(P<0.05); there were statistical significance of the high-dose ethanol group at1h,2h,4h and8h compared to control group(P<0.05); there were statistical significance of the high-dose ethanol group at1h,2h,4h and8h compared to medium group(P<0.05).The intensity of p-ERK1/2in amygdale of the control group was higher than mediorsal nucleus.The expression of p-ERK1/2in amygdale of the low-dose ethanol group was increased. There were statistical significance of the low-dose ethanol group at2h compared to control group(P<0.05). There were statistical significance of the medium-dose ethanol group at1h,2h,4h and8h compared to control group(P<0.05);there were statistical significance of the medium-dose ethanol group at1h,2h and4h compared to low group(P<0.05); there were statistical significance of the high-dose ethanol group at1h,2h,4h and8h compared to control group(P<0.05);there were statistical significance of the high-dose ethanol group at1h,2h and4h compared to medium group(P<0.05).3. the results of Western-blot:The intensity of ERK1in cerebral cortex of the high group did not change significantly, almost24hours without changing in the total. There were statistical significance of the high-dose ethanol group at1h,2h and4h compared to control group; There were statistical significance of the medium-dose ethanol group at2h compared to control group; There were statistical significance of the2h of the high-dose ethanol group at cerebral cortex, Hippocampus and nuclear groups of midbrain compared to control group, There were statistical significance of the cerebral cortex and nuclear groups of midbrain compared at control group.Conclusion:1. The expression intensity of ERK1/2phosphorylation in brain nerve cells which induced by ethanol is associated with the concentration and intake of ethanol.2. The ethanol selectively affected the rats’ cerebral cortex, Hippocampus and nuclear groups of midbrain. And the changes are related with the intake of ethanol. |
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