Inhibitory Effect Of Salidroside On Proliferation In Human Gastric Carcinoma Cells Sgc-7901and The Preliminary Study Of Its Anti-tumor Effect

OBJECTIVE:To investigate the inhibiting effect of salidroside on human gastric carcinoma cells SGC-7901and its mechanism of anti-tumor.METHODS:Human gastric carcinoma cells SGC-7901were divided into four groups:0.5mg/ml salidroside group,1.0mg/ml salidroside group,2.0mg/ml salidroside group and control group. The proliferation of cells were assayed by MTT assay and argyrophilic staining (AgNORs). Flow cytometry was used to assess the cell cycle progression and apoptosis. The migration activity of gastric carcinoma cells SGC-7901was detected by cell scratch assay.RESULTS:MTT assay results showed that the the salidroside drug concentration group can inhibit the growth and proliferation of gastric carcinoma cells SGC-7901, and the inhibition rate was significantly higher than control group (P<0.05). argyrophilic staining experiments showed that: AgNORs values in salidroside group (0.5mg/ml、1.0mg/ml、2.0mg/ml) were (2.91±0.74)/nuclear、(2.35±0.48)/nuclear and (1.68±0.67)/nuclear, which significantly lower than those in the control group (3.80±0.1)/nuclear. proliferation of gastric carcinoma cells SGC-790was inhibited (p<0.05). In salidroside groups, the proportion of gastric carcinoma cells SGC-7901in S phase was lower and the proportion of cells in G2/M phase was higher than that in control group (p<0.05). The apoptotic rates of gastric carcinoma cells SGC-7901in0.5mg/ml salidroside group,1.0mg/ml salidroside group,2.0mg/ml salidroside group were (25.95±0.10)%、(29.64±0.11)%、(31.72±1.18)%, which were higher than that in control cells (8.20±0.10)%(p<0.05). The relative migrations of gastric carcinoma cells SGC-7901which were incubated with0.5mg/ml,1.0mg/ml,2.0mg/ml salidroside after48h were(52.00±1.04)%、(38.08±2.01)%、(16.00±1.41)%, which were less than that of control cells(67.59±1.45)%(P<0.05)CONCLUSION:salidroside could effectively inhibit growth and proliferation of gastric carcinoma cells SGC-7901, and arrest cell cycle and induce apoptosis and reduce the cells’migration. The inhibiting effect of growth and proliferation of salidroside on human gastric carcinoma cells SGC-7901, enhanced with the increase of drug concentration and time.