| Background Previous studies demonstrate that dragon’s blood has the characteristics of promote the wound healing, anti-platelet aggregation, anti-inflammatory analgesic, anti-bacterial and antioxidant and other biological activity.Objective To find out the effectiveness of dragon’s blood for treating wound and identify the effects of dragon’s blood on the expression of substance P (SP), TGF-β1and Bcl-2in wound tissue of diabetic scalded rats.Methods A total of112Wistar rats were randomly divided into four groups:dragon’s blood group, silver sulfadiazine (SD-Ag) group, diabetic control group and normal control group. Diabetic deep Ⅱ degree burned models were established in dragon’s blood group, SD-Ag group and diabetic control group, and the normal deep Ⅱ degree burned model were established in normal control group.After1,3,5,7,11,15and21days each rat was calculated the wound healing rate of skin tissue and the expression level of substance P(SP), TGF-β1and Bcl-2. Results On the7th day post scald, the wound healing rates of dragon’s blood group and normal control group were higher than the of SD-Ag group and diabetic control group (P<0.05). After21days, the wound healing rate of dragon’s blood group was significantly higher than that of SD-Ag group and diabetic control group (P<0.05) and highest in normal control group. After15days, the positive expression level of SP reached a peak in normal control group and was higher than other three groups, and then decreased gradually; The expression was most positive in dragon’s blood group, SD-Ag group and diabetic control group at the21th day post scald, and which was significantly higher in dragon’s blood group (P<0.05). On the third day after burned TGF-β1expressed weakly in dragon’s blood group and normal control group, the expression was strongest and significantly higher than SD-Ag group and diabetic control group on the7th day (P<0.05), which gradually declined after11th day. It began to appear in SD-Ag group and diabetic control group on the5th day and reached the peak on the11th day, but there was no significant difference between the two groups (P>0.05). On the third day after scald, the expression level of Bc1-2in the normal control group was significantly higher than that in other groups. From the fifth day, the expression of Bcl-2was gradually increased in each group and reached to peak, and highest in dragon’s blood group (P<0.05), but there was no significant difference between dragon’s blood group and normal control group (P>0.05). Conclusion Dragon’s Blood can significantly promote the healing of diabetic burned wounds significantly, and regulate the expression of SP, TGF-β1and Bcl-2effectively in the process of wound healing. |
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