| Objective:Continued to give taurine treatment after acute spinal cord injury. Expiore the effectiveness of taurine in the spinal cord injury repair through observing the expression of caspase-3and PCNA, the improvement of exercise capacity in rats and the pathological changes.Method:84adult male Sprague-Dawley rats weighting about300-350g wererandomly divided into2groups:(1) Control group (group A, n=42);(2) Taurine treatment group (group B,n=42).The control group and the taurine treatment group were divided into7subgroups at3h,6h,12h,1d,4d,1d,14d in accordance with the executed time. The model of Spinal cord injury was established according to the improved Allen method. Spinal cord to combat the range of the T11, T10and T9.300mg/kg of taurine was used to the taurine treatment group intraperiton-eally, the control group were treated with the same volume of saline vehicle(NS) immediately after injury. Each model was given the same dosage once a day to the3h,6h,12h,1d and4d group or lasted for6days following the injury to the7d and14d group. Observe the following indicators in the successful construct-ion of model:(1)Combined behavioral score (CBS) was used to evaluate the spinal nerves function each time point after the operation.(2)Pathological changes were observed in rat spinal cord of each subgroup by HE staining and Nissl staining.(3)Observed in rat spinal cord of each subgroup of caspase-3and PCNA expression level by immunohistochemical and two groups compared.Results1. Taurine can protect nerve dysfunction caused by spinal cord injury and to facilitate the repair of spinal cord injury, taurine treatment group combined behavioral scores significantly lower than the control group at7d and14d there was a significant statistically difference between them(P<0.05).2. Taurine treatment in all aspects of pathology evaluation results more excellent than the control group of the same point in time when the observed damage to the spinal cord with HE staining and Nissl staining.3. PCNA expression in the control group (group A) spinal cord injury in3h, the7d of its expression reached a peak and then begin to decline. No significant difference in the expression of PCNA when compared taurine treatment group (group B) rats with the control group (group A) rats were the same in3h, the4d of its expression reached a peak and then begin to decline. PCNA expression between two groups of rats was statistically significant (P<0.05) in6h,12h,1d,4d,7d and14d.4. caspase-3expression in the control group (group A) spinal cord injury in3h,the4d of its expression reached a peak and then begin to decline.The positive expression ofcaspase-3in the3h taurine treatment group (group B) rats was weaker than the control group (group A) rats, the7d of its expression reached a peak and then begin to decline, caspase-3expression between two groups of rats was statistically significant(P<0.05) in4d and7d.Conclusion:1. Taurine can reduce secondary damage after spinal cord of acute spinal cord injury, promote recovery of neurological function.2. Taurine promote DNA repair and reduced apoptosis by inducing cell nuclear antigen (PCNA) increases and caspase-3protein down to complete. Taurine reduce the harm of secondary spinal cord injury. |
PDF Full Text Request