The Experimental Research On The Neurocyte Apoptosis And The Relatived Proteins Expression Following Acute Spinal Cord Injury In Rats Epidural Injected By Erythropoietin

Erythropoietin(EPO),34kd,is a sialoglycoprotein. EPO was considered to be excreted only by the kidney and responsible for the proliferation,maturation,and differentiation of the precursors of the erythroid cell line incretory hormone. Recent studies have demonstrated that,in addition to kidney,liver,lung,spleen,Placenta and Procreative organ can excrete EPO. EPO and its receptors (EPOR) are widely expressed in the central nervous system(CNS). EPO,a multifunctional nutritional factor and neuroprotective factor,which can modulate the inflammatory and immune response,not only plays a crucial role in the regulation of erythropoiesis,but also plays a crucial role in the regulation neurodevelopment,and have neuroprotective function in different conditions of neuronal damage.The experimental study is to explore the effect about rhEPO on neuromotor function, secondary pathology change and the expression of apoptosis neural cells and the relatived proteins caspase-3,fasl, after rhEPO was epidural administered in the traumatic spinal cord injury rats at different time. The experimental study is devided into three parts:The first partThe experimental research on the neuromotor function recovery of rat after which was epidural injected by rhEPOObjective To explore the Protective protect effect of rhEPO on neuromotor function of rat and observe the neuromotor function recovery of rat, after which was epidural injected in traumatic spinal cord injury rat at different time.Method A total of 48 adult male Sprague-Dawley rats weighting (270±10) g were randomly divided into four groups. Rats in normal group(n=6),in a sham group (n=6) underwent laminectomy producure only.In a control group, rats (n =18) received normal saline epidurally respectively at 1h,6h,24h after injury. Treatment groups (n =18) received 5000iu/kg body weight of recombinant humane erythropoietin administered epidurally after respectively at 1h,6h,24h after injury. The SCI was induced with 70g/cm impact according to the improved Allen method . Behavioral evaluation of the rats was made 48 hours after trauma by using the Basso,Beattie, and Bresnahan (BBB) scoring system and Rivlin's tiltboard experiment;Result Compared to concurrent control groups, neuromotor function are significantly improved in 1h 6h and 24h experiment groups. The angles of Rivlin's tiltboard experiment and the scores of BBB are all significantly increased(p<0.05).Conclusion: Analysis of the results indicated that early application of rhEPO could significantly lessen the lesion of neuromotor function.The second partProtective effect of Erythropoietin on the Neurocyte apoptosis following experimental acute spinal cord injury in ratsObjective To explore the Protective protect effect of rhEPO on neuromotor function and observe the expression of neural cells apoptosis, after which was epidural injected in traumatic spinal cord injury rat at different time.Method A total of 48 adult male Sprague-Dawley rats weighting (270±10) g were randomly divided into four groups. Rats in normal group(n=6),in a sham group (n=6) underwent laminectomy producure only.In a control group, rats (n =18) received normal saline epidurally respectively at 1h,6h,24h after injury. Treatment groups (n =18) received 5000iu/kg body weight of recombinant humane erythropoietin administered epidurally after respectively at 1h,6h,24h after injury. The SCI was induced with 70g/cm impact according to the improved Allen method . Behavioral evaluation of the rats was made 48 hours after trauma by using the Basso,Beattie, and Bresnahan (BBB) scoring system and Rivlin's tiltboard experiment;Hematoxylin and eosin staining was performed for histological observation after injury.Injured spinal cord tissues cells apoptosis were examined by the terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end labeling(Tunel)reaction at 48 hours;Result Compared to concurrent control groups, neuromotor function are significantly improved in 1h 6h and 24h experiment groups. The distroyed extent of the spine cord are more seriously in control groups than with HE histology stain. The number of TUNEL-positive cells was significantly lower in the erythropoietin-treated than in the saline-treated groups (P < 0.05).Conclusion: Analysis of the results indicated that early application of rhEPO could lessen the lesion of neuromotor function and inhibt apoptosis.The protection is partially due to the reduction of neural cells apoptosis.Protective effect of erythropoietin by epidural injection on the neurocyte apoptosis and the relatived proteins following experimental acute spinal cord injury in ratsObjective To explore the protective protect effect about rhEPO on neuromotor function, secondary pathology change and the expression of apoptosis neural cells and the relatived proteins caspase-3,fasl, after rhEPO was epidural administered in the traumatic spinal cord injury rats at different time.Method A total of 48 adult male Sprague-Dawley rats weighting (270±10) g were randomly divided into four groups. Rats in normal group(n=6),in a sham group (n=6) underwent laminectomy producure only.In a control group, rats (n =18) received normal saline epidurally respectively at 1h,6h,24h after injury. Treatment groups (n =18) received 5000iu/kg body weight of recombinant humane erythropoietin administered epidurally after respectively at 1h,6h,24h after injury. The SCI was induced with 70g/cm impact according to the improved Allen method . Behavioral evaluation of the rats was made 48 hours after trauma by using the Basso,Beattie, and Bresnahan (BBB) scoring system and Rivlin's tiltboard experiment;Injured spinal cord tissues cells apoptosis were examined by the terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end abeling (Tunel) reaction at 48 hours;Fasl and Caspase-3 expression was determined by immunohistochemical analysis at 48 hours after injury too. The results were observed by light microscope and analysised by SPSS statistics soft.Result Compared to concurrent control groups, neuromotor function are significantly improved in 1h 6h and 24h experiment groups. The indexes of neural cell decreased significantly (P < 0.05)too. FasL and Caspase-3 expressed less in experiment groups, The number of neural cells correlated in a positive linearity with the number of Caspase-3(r=0.941)and Fasl ( r = 0. 929) expressed cells.(p<0.01)Conclusion Analysis of the results indicated that apoptosis plays an important role in the secondary spinal cord injury;Activation of Fasl and Caspase-3 is related to cells apoptosis in the injured spinal cord. Early application of rhEPO could lessen the lesion of neuromotor function. The protection is partially due to the reduction of neural cell apoptosis.