Study On The Applications Of Resonance Light Scattering Technique In Protoberberine Alkaloids Analysis

The resonance light scattering (RLS) technique is developed from the1990s. For its advantages of simple operation, fast response, high sensitivity and it can be completed by ordinary fluorescence spectrophotometers, RLS technique has been arrested extensive attention by analysts. The RLS technique has been used in many fields of analytical research work, such as life sciences, environmental science, materials science and pharmaceutical analysis. In this paper, it has been applied to the analysis of berberine drugs contents and their corresponding RLS spectra have been studied. The main contents are as follows:(1) A novel RLS detection method for Berberine Hydrochloride (BH) is established based on the enhanced resonance light scattering signals at290nm which result from BH interacting with Perfluorooctanesulfonate (PFOS) in Britton-Robinson (BR) buffer medium. The enhanced IRLS is proportional to the concentration of BH in a range of0.044-7.8μg/mL. The limit of detection (3a) is4.4ng/mL. This method was successfully applied for the determination of BH in tablets with relative standard deviation (RSD)≤4.37%.(2) For the determination of Palmatine Hydrochloride (PaH), a novel RLS method has been developed based on the largely enhanced RLS signals at297nm due to the interaction between Silicotungstic acid (STA) and PaH by electrostatic force in the pH0.91HCl-NaAc medium. The linear range of PaH is0.091～.2μg/mL. The limit of detection (3σ) is9.1ng/mL. In this work, the effects of pH and ionic strength on interaction were investigated. At the same time, the UV spectra, the fluorescence spectra and the reaction mechanism of system were also investigated. This method has been applied successfully to the determination of PaH in pharmaceutical and blood serum samples with relative standard deviation (RSD)≤4.2%. (3) Single stranded poly(A), as a kind of Adenine-rich nucleic acids, could stabilize13nm gold nanoparticles (AuNPs) in high ionic strength. It found that Palmatine Hydrochloride (PaH) is strong affinity to poly(A). When poly(A) exposed to PaH, aggregation of AuNPs could be found, which results in the enhanced RLS signals. A novel RLS method for determining PaH has been developed based on the enhanced intensity of RLS with a PaH range of0.023～2.5μg/mL. The limit of detection is2.3ng/mL. The absorption spectra and color changing of PaH were also studied in this work. The reaction mechanism of this system have been further investigated by Scanning Electron Microscope (SEM), Dark-field Light Scattering Images (DLSI), Dynamic Light Scattering (DLS) and Circular Dichroism (CD). The RLS method has been applied successfully for the determination of PaH in pharmaceutical and samples of urine, relative standard deviation (RSD)≤4.0%.