The Expression Of The Notch1and The Mutation Of Th E Notch1in Ptcl

【Background】Peripheral T cell lymphoma (PTCL), a kind of malignanthematologic tumors, and the acute T cell leukemia (T-ALL), are both derivedfrom the T progenitor cells. PTCLs are common in children and adolescents, andalso can be found in adult patients. PTCL is a highly heterogeneous tumor onpathology, it is a invasive disease, and has a poor prognosis [1-4]. So manystudies have explored its prognostic factors, but there were no same results. Atpresent, the general opinion is that the prognosis of PTCL is poorer than B celllymphoma. But as a result of PTCL is a heterogeneous group, we should analyzeit based on different types of it, and it is important to judge its prognosiscomprehensively combing with other prognostic factors. The pathogenesis ofPTCL is still not clear. And we have no standard treatment regimen yet. Over theyears the molecular mechanisms and molecular signaling pathway for malignantdisease derived from the T progenitor cells has laid a foundation of themolecular targeted therapy, which includes: Notch1Signaling pathway, NF-ΚBsignaling pathways, cell surface antigen (CD30, CD52, CD4), Ron tyrosinekinase,and so on. In recent years, many Hematology researchers focu on theNotch1Signaling Pathway in T cells malignant hematologic tumors. Aberrantactivation of the Notch1Signaling pathway is not only been proved to play akey role in the formation and prognosis of the acute T cell leukemia, also in T cell leukemogenesis and Prognosis. Gain-of-function mutations of the NOTCH1geneHD-N、HD-C、TAD、PEST domain cause aberrant activation of the Notch1Signaling pathway.With the low incidence of the peripheral T cell lymphoma,distinct from the acute T cell leukemia (T-ALL), the research of Notch1Signaling pathway in this disease is still few. So,in our experiment, we detectedthe expression of active forms of Notch1(NICD) protein and analysis mutationof NOTCH1gene HD-N, HD-C, TAD, PEST fragments about the peripheral Tcell lymphoma.【Objectives】To detecting the expression of activate type of Notch1(NICD) protein in peripheral T cell lymphoma tissue, and analysis of NOTCH1gene mutation.【Methods】(1) collection of20cases of peripheral T cell lymphoma(13cases,7cases of PTCL NOS ALCL),5cases of patients with reactive hyperplasia tissues.(2) though the method of immunohistochemistry to detecte the expression of reactive hyperplasia lesion tissue activation of Notch1(NICD) antibody in20cases of peripheral T cell lymphoma and5cases patients with reactive hyperplasia tissues (3) analysis the mutation by SPSS of the HD-N, HD-C, TAD, PEST fragment of the NOTCH1gene.【Results】(1) collected pathological tissue section of20cases of lymphoma patients and5cases of reactive lymphoid hyperplasia.(2)19patients with peripheral T cell lymphoma activated Notch1(NICD) antibodyexpression were positive, another one PTCL patient and5cases of reactive lymphoid hyperplasia tissue expression were negative.12cases of PTCL NOS were higher expression than the anthor group of7cases of ALCL.(3) SSCP analysis show that peripheral T cell lymphoma have NOTCH1gene mutation in HD-C fragment. 【Conclusion】Peripheral T cell lymphoma express aberrant activatedNotch1antibody, and mutation of NOTCH1gene may cause the abnormalexpression of activated Notch1.