MicroRNA-26b Inhibits The Invasion Of HCC Cells Via Regulating The Notch1 Signalling Pathway

Objective: Hepatocellular carcinoma(HCC)is a malignant tumor with the fourth highest incidence rate and the second highest lethality rate in China,posing a serious threat to the lives and health of our people.Although great progress has been made in the treatment of HCC,the overall prognosis is still poor.The reason is related to the high invasiveness and early metastasis of HCC.In recent years,more and more studies have confirmed that mi RNAs are abnormally expressed in the tissue cells of malignant tumors,and that their role in regulating the occurrence and development of various tumors is crucial.Therefore,this study mainly explores the mechanism of mi R-26 b involved in the invasion of primary hepatocellular carcinoma(HCC).Methods: The human liver cell lines HHL-5 and HCC cell lines Hepb-3,Hu H-7,MHCC97-L,MHCC97-H were cultured in cell culture medium.Real-time fluorescent quantitative PCR(q RT-PCR)was used to detect the expression level of mi R-26b;mi R-26 b mimics,mi R-26 binhibitor,and si Notch1 were used to transfect HCC cells;MTT experiments were used to detect the viability of HCC cells after transfection;Western blot was used.Changes in protein expression levels of the Notch1 receptor were detected;transwell cells were used to determine the invasive capacity of HCC cells after different treatments.Results: The relative expression levels of mi R-26 b in human normal liver cell line HHL-5 and HCC cell lines Hepb-3,Hu H-7,MHCC97-L,MHCC97-H decreased in turn with the increase of their invasive ability;Inhibiting the expression of mi R-26 b,the protein expression of Notch1 receptor was significantly increased,and the invasiveness of HCC cells was significantly enhanced;In contrast,when the expression of mi R-26 b is upregulated,the protein expression of Notch1 receptor is significantly reduced,while the invasiveness of HCC cells is significantly reduced;mi R-26 b may regulate the invasion of HCC cells by regulating Notch1 signaling pathway.Conclusion: mi R-26 b inhibits the invasiveness of HCC through negative regulation of Notch1 signaling pathway,laying a theoretical foundation for the mechanism of HCC metastasis.mi R-26 b may become a new target for the treatment of primary hepatocellular carcinoma.