The Effect Of5-amino Salicylic Acid On The Proliferation, Apoptosis And Cell Cycle In Ht-29Cell Line

Objects: To investigate the effect of5-amino salicylic acid (5-ASA) on the cellproliferation, apotosis, cell cycles,and the expression of AuroraB and BubR1inhuman colorectal cancer line HT-29.Methods: The HT-29cell were co-cultured with0mM(Group A),10mM(Group B),20mM(Group C),30mM(Group D),40mM(Group E) of5-amino salicylic acid（5-ASA） respectively. The proliferation, apotosis and the cell cycles wererespectively detected by CCK-8, TUNEL and the flow cytometry. The expressions ofAuroraB and BubR1were detected by immunocytochemistry.Every test was repeatfive times and the mean value was calculated.Result:（1）The inhibitory rates(%) of cell proliferation in the4group（sGroup B～Group E）were8.20±3.03,14.03±2.41,21.05±3.33and25.59±3.90respectively, amongwhich there was a significant difference（p<0.05）.（2）The cell apotosis indexs(%) in the5groups(Group A～Group E)were0.27±0.38,7.11±1.65,7.87±1.28,10.63±2.31and14.82±2.93respectively, among whichthere was a significant difference（p<0.05）.（3）The G0/G1fractions (%) in the5groups(Group A~Group E)were86.13±2.50,85.46±4.33,83.57±2.84,76.80±2.10and62.28±3.05respectively, and that inthe Group D or in Group E was significantly different from the other groups(p<0.01).The S fractions (%) in the5groups(Group A~Group E) were10.43±1.22,10.80±3.51,11.45±3.34,11.05±2.11and18.89±1.68respectively，and that in the Group Ewas significantly different from the other groups (p<0.01). The G2/M fractions (%) inthe5groups (Group A~Group E) were3.44±1.36,3.74±1.08,4.98±1.13,12.15±2.04and18.83±1.87respectively，and that in the Group D or in Group E wassignificantly different from the other groups(p<0.01). (4) The mean optical density (MOD) of AuroraB expression byimmunocytochemistrical stain in the5groups were0.78±0.03,0.55±0.03,0.53±0.04,0.47±0.03and0.36±0.02respectively, among which there was a significantdifference（p<0.01）. The mean optical density (MOD) of BubR1expression in thefive groups were0.31±0.01,0.26±0.02,0.26±0.01,0.24±0.02and0.22±0.01respectively, among which there was a significant difference（p<0.01）.The MOD ofAuroraB and BubR1expression were negatively correlated with the concentration of5-ASA(p=0.00）. They also showed a positive correlation with the G0/G1fraction(p=0.00）, and a negative correlation with the G2/M fraction(p=0.00）.Conclusion:A certain dosage of5-ASA could dosage-dependently inhibit the cell proliferation,reduce the G0/G1cell, and promote the cell apoptosis in HT-29cell, which showed adecreased expression of AuroraB and BubR1.