Testing And Evaluation On Enhancing Immune Function Of Two Kinds Of Ganoderma Lucidum Prescription Products

Ganoderma lucidum spore is the germ cell of Ganoderma lucidum with allgenetic active materials. The main effective components include polysaccharides,triterpene and peptide, also are rich in amino acids, sugar, fatty acid and traceelements. Artificial cordyceps and natural cordyceps has similar pharmacologicalcomposition, the main effective components include adenosine, urine glucoside, urinepurine, birds glucoside, mannitol, ergosterol sterol, polysaccharide, amino acids, traceelements, and vitamin B1, B2, etc. Soybean isoflavone is a kind of natural existentphytoestrogens, extracted from the legumes, has a variety of physical activity.Objective: To explore the enhanced immune function on mice of ganodermalucidum cordyceps prescription and ganoderma lucidum soyisoflavone prescriptionfrom the point of view of the complex. Methods: According to “Evaluating Rules andTest Methodology of The Function of foods of Health Care” and“Pharmacologicalexperimental Methodology”, female K Mmouse was used in this study, divided intofour groups at random: test group fed with low dose of the two prescription(75mg/kg.bw); test group fed with middle dose of the two prescription(150mg/kg.bw);test group fed with high dose of the two prescription(450mg/kg.bw); and controlgroup fed with the same volumed distilled water. Each group was fed for28days, during the days, the weight of each mouse was measured every7days and the volumeof the feed for each mouse was changed according to the weight. On the28th day, allthe mice of4groups were killed to make the following tests: immune organ index test,delayed type hypersensitivity test, lymphopoiesis of mice induced by ConA, serumhemolysin test, carbon clearance capability test, macrophage phagocytosis of chickenred blood cells capability test. They were applied to evaluate the effects of ganodermalucidum cordyceps prescription and ganoderma lucidum soyisoflavone prescription onthe immune regulation of mice.Results:(1)Low dose of ganoderma lucidum cordyceps prescription had noeffects on immune organ index， proliferation of splenic lymphocytes and serumhemolysin level，but contributed to delayed type hypersensitivity, carbon clearancecapability and phagocytosis of macrophages. Low dose of ganoderma lucidumsoyisoflavone prescription had no effects on immune organ index, delayed typehypersensitivity, proliferation of splenic lymphocytes and serum hemolysin level, butcontributed to carbon clearance capability and phagocytosis of macrophages.(2)Middle dose of ganoderma lucidum cordyceps prescription had no effects onimmune organ index, proliferation of splenic lymphocytes and serum hemolysin level,but can significantly enhanced delayed type hypersensitivity, carbon clearancecapability and phagocytosis of macrophages. Middle dose of ganoderma lucidumsoyisoflavone prescription had no effects on immune organ index and serumhemolysin level, but contributed to delayed type hypersensitivity, proliferation ofsplenic lymphocytes carbon clearance capability and phagocytosis of macrophages.(3)High dose of ganoderma lucidum cordyceps prescription had no effects ondelayed type hypersensitivity, serum hemolysin level, carbon clearance capability andphagocytosis of macrophages, but can significantly enhanced immune organ indexand proliferation of splenic lymphocytes. High dose of ganoderma lucidumsoyisoflavone prescription had no effects on immune organ index, delayed typehypersensitivity, proliferation of splenic lymphocytes, serum hemolysin level,carbon clearance capability and phagocytosis of macrophages.Conclusion: Proper dose of either ganoderma lucidum cordyceps prescription or ganoderma lucidum soyisoflavone prescription can enhance the non-specificimmunity and specific immunity of the organism. It’s beneficial to defense the attackof harmful factors and to prevent the genesis and development of chronic diseasesassociated with immune injury. ganoderma lucidum, cordyceps and soyisoflavone canact as the ingredients of hygienical foods that can enhance the immunity of the body.This study provides proofs from the laboratory for the applications of ganodermalucidum, cordyceps and soyisoflavone in the fields of exploitation of hygienical foodsand medicines.