Application Of Salidroside Protect Renal Ischemia-Reperfusion Injury In Rats

Objective To study the different doses of salidroside protective effect in rat renal ischemiareperfusion injury, determine salidroside can reduce renal ischemia reperfusion injury in time limitand explore its mechanism of action.Methods The48healthy SD rats were randomly divided intosix groups, namely salidroside5mg/kg group,10mg/kg group,20mg/kg group,40mg/kg group,Operated group and control group. Through the removal of the right kidney, left renal pedicleclamping for45minutes to establish renal ischemia-reperfusion model. Were measured in serumcreatinine (Scr), blood urea nitrogen (Bun), normal kidney and renal pathology in the expression ofHsp72. Take another54healthy SD rats were randomly divided into nine groups, namely blankcontrol group (n=6), ischemia-reperfusion group by clamping15,30,45and60minutes group (n=6), Intraperitoneal injection of salidroside solution group by clamping15,30,45and60minutesgroup (n=6).Result Comparison group of salidroside5mg/kg,10mg/kg group,40mg/kg groupand Operated group, the level of salidroside20mg/kg group Scr, Bun levels were significantlydecreased (P <0.01), significantly reduced pathological damage, tissue expression of Hsp72alsodecreased significantly. Compared with the control group, salidroside20mg/kg group Scr levels,Bun level increased slightly, less pathological damage.Comparison with ischemia and reperfusionin each group, the salidroside each group Scr level, Bun levels, significantly reduced pathologicaldamage, kidney Hsp72and NF-κB expression was significantly reduced. Compared with thenormal control group, the salidroside each group Scr level, Bun levels, has increased, includingclipping60minutes significantly increased the pathological damage, clipping15,30and45minutes is not obvious.Conclusion Determine the concentration of20mg/kg of salidroside is thebest protection.Application of salidroside after renal ischemia-reperfusion injury significantlyreduced, the mechanism and enhance the antioxidant capacity of the kidneys, reducing kidneytissue inflammatory cell infiltration and inhibition of Hsp72and Nf-κB expression in renal tissue, where the longest renal ischemia time is45minutes.