The Role Of SDF-1Receptor CXCR7in Proliferation, Adhesion And Invasion Of Hela Cells

Cervical cancer is one of the global high-risk female malignant tumors, which isthe second incidencial cancer, just after breast cancer. The prognosis of cervical cancerpatients is bound up with tumor invasion and transfer, distance metastasis has becomethe main obstacle to improve the survival rate of patients. So, investigating themolecular mechanism of cervical cancer metastasis and exploring the potential targetcan cure cervical cancer both have important realistic meaning. SDF-1(stromalcell-derived factor1)and its receptor plays an important role on the regulation in thecourse of tumorigenesis and development. SDF-1had been considered to mediatebiological process through its unique receptor CXC chemokine receptor4(CXCR4) formany years. However, Recent studies reported that SDF-1was also a ligand of a novelchemokine receptor-CXC chemokine receptor7(CXCR7), which is expressed in avariety of tumor cells. It was confirmed that CXCR7plays an important role in invasionand metastasis of several types of tumor induced by SDF-1. although its specificfunctions to various research models have some differences. Recent studies reportedthat CXCR7was also expressed in HeLa, but the function of which remains unclearnow. This work takes cervical cancer cells as the research object, and uses smallmolecular antagonists of CCX733and AMD3100inhibition processing in vitro toinvestigate the influence of CXCR7on HeLa proliferation，adhesion and invasionabilities induced by SDF-1, clarifying the function of SDF-1/CXCR7biological aix inthe course of cervical cancer metastasis to provide a new target for cervical cancertreatment. The main research and results are as follows:①In present study, the expressions of CXCR4and CXCR7on HeLa cells weredetected by Western blotting. Results revealed that: both CXCR4and CXCR7areexpressed on HeLa cells.②Effects of CXCR4and CXCR7on cell behaviors were tested by blocking withtheir antagonists, respectively. Cell proliferation, cell adhesion and cell invasion wereevaluated by MTT, adhesion assay, transwell assay, respectively. Gelatin zymographywas used to detect the activity of gelatinase in HeLa cells induced by SDF-1. Resultsrevealed that: The proliferation, adhesion and invasion of HeLa cells are significantlyincreased by SDF-1and it can improve the ability of HeLa cells to secrete MMP-2. Theproliferation, invasion and adhesion of HeLa cells induced by SDF-1were inhibited by CXCR4blockage or CXCR7blockage.These results suggested that: both CXCR4and CXCR7are expressed on HeLacells. The proliferation, adhesion and invasion of HeLa cells induced by SDF-1wereinhibited by CXCR4blockage or CXCR7blockage. CXCR7can mediate proliferation,adhesion and invasion of HeLa cells induced by SDF-1,which indicated that CXCR7isa potential target for cervical cancer therapy.