Relationships Between Concentrations Of Free Fatty Acid In Serum And Oxidative Damage Levels In Placentalmitochondria And Preeclampsia

ObjectiveThe purpose of this study was to investigate the relationships betweenconcentrations of free fatty acid(FFA)in maternal serum and oxidative damage levelsin placental mitochondria and preeclampsia.MethodsA total of60women with preeclampsia and60normal pregnant women ascontrol participated in this study.All are admitted to Fujian Maternity and Child HealthHospital for delivery from August2010to May2011.Patients with preeclampsia weredivided into early-onset group(n=30,presented at≤34weeks of gestation)andlate-onset group(n=30，presented at＞34weeks of gestation),with30normal pregnantwomen as early control group(≤34weeks of gestation)and30as late controlgroup(＞34weeks of gestation). Improved copper agent colorimetry was used todetect FFA in maternal serum.Ultraviolet colorimetry was used to detect glutathioneperoxidase(GSH-PX)and catalase(CAT)activity in maternal placenta and malondiaidehyde(MDA)and permeability transition(PT)pore in palcental mitochondr-ia.WSTwas used to detect superoxide dismutase(SOD)activity in placenta. High PerformanceLiquid Chromatography(HPLC)technique was used to detect the concen-tration ofCoenzymumQ10（CoQ10） in placenta.Fluorescent dye was used to detectmitochondrial membrane potential in palcenta.Real-time fluorescent quantitative PCRwas used to detect mitochondrial DNA(mtDNA)expression in palcenta.Results①Maternal serum FFA was (1.58±0.54)mmol/L in early-onset PE group and (1.51±0.36)mmol/L in late-onset PE group，significantly elevated as compared to(1.03±0.51)mmol/L in early control group and (0.92±0.50)mmol/L in late control group.However，no significant difference was found between early-onset and late-onsetpreeclampsia groups.②Mean placental GSH-PX,CAT and SOD activity were significantly decreased in the early-onset PE group and late-onset PE group as compared to late controlgroups，respectively.③Mean placental CoQ10were significantly increased in the early-onset PE groupand late-onset PE group as compared to late control groups,respectively.④Mean palcental mitochondria MDA were significantly elevated in the early-onsetPE group and late-onset PE group as compared to late control groups. Meanabsorption value that present the permeability of palcental mitochondriapermeability transition were significantly elevated in the early-onset PE group andlate-onset PE group as compared to late control groups.Mean palcental mt DNAexpression were significantly elevated in the early-onset preeclampsia group andlate-on set PE group as compared to late control groups.⑤Mean palcental mitochondria MDA concentration correlated positively withmaternal serum FFA concentrations；and negatively with placental antioxidantenzyme.Conclusions①Increased FFA in maternal serum may be involved in the pathogenesis ofpreeclampsia.②High levels of oxidative damage in placental mitochondria may be involved in thepathogenesis of preeclampsia.③Increased FFA in maternal serum may contribute to high oxidative damage levelsin placental mitochondria in women with preeclampsia.④Decreased activity of antioxidant enzyme in placenta may contribute to highoxidative damage levels in placental mitochondria in women with preeclampsia.