Expresson And Significanse Of Calreticulin And Galectin-3 In Endometrium Of Polycystic Ovarian Syndrome In Rat Model

Objective To investigate the expression and biological significance of CRT and Galectin-3 in the endometrium of polycystic ovarian syndrome ( PCOS) in rat model. Methods Sixty rats were randomly divided into two groups : PCOS group ( n =30) and control group ( n = 30) . The rats in the experimental group were given subcutaneous implanting of levonorgestrel silica gel staff and injected 1.5 I U hCG twice daily for 9 days from the 4th day. The rats in the control group were injected with normal saline at the same time. Ovarian morphologic changes, sex hormone levels were detected. Results The weight and volume of ovarian in the model rats were higher than that in the control rats′( P < 0 . 01) . The rats in model group had similar pathological appearance to polycystic ovary. The multiple follicular cysts, and the number of thecacells and interstitial cells increased in the ovaries of modeling group showed . There was more developed follicles and corpus lutea. The progesterone, testosterone, luteinizing hormone, fasting insulin, glucose the LH /FSH ratio and HOMA index were significantly higher in experimental group than those in control group, so as . There was the expression of protein CRT and Galectin-3. The CRT and Galectin-3 protein expressed in endometrium in PCOS group and control group. The expression of CRT and Galectin-3 of endometrial in PCOS rats were significantly lower (P < 0.01) than those in control group. Conclusion The rat model induced by levonorgestrel with hCG was proved to be an ideal PCOS animal model. CRT and Galectin-3 may be closely correlated with the pathogenesis of PCOS.