Chemical Compositions And Allelopathy Of Branch And Leaf Of Rosa Damascena Planted In Fangshan Beijing

As a high-quality rose variety in the world, a large area of Damascus rose has been brought in and cultivated in many parts of China. In the process of rose cultivation management, resource waste and environmental pollution result from pruning each year. In order to explore the utility value of abandoned leaves and improve the level of rose cultivation management, this study investigated soluble polysaccharide of rose leaves and branches, protein, micronutrient elements, extraction of plant polyphenols and the conditions of content determination. And through the application of the best experiment condition, the study analyzed the soluble polysaccharide, protein, micronutrient elements, and the content of polyphenols in rose leaves and branches in different growth periods and different physiological conditions (flowering and non-flowering). Besides, It also explored the allelopathy of Damascus rose leaves and branches water immersion. The results showed that:The nutritive substance content of polysaccharide, protein and Mineral elements in leaves is obviously higher than that in branches; the nutritive substance content in flowering leaves and branches is obviously higher than that in non-flowering ones; in different growing periods during the growing season, Damascus roses’ polysaccharide content presented a V-change trend, while soluble protein content presented a∧-change trend, and dynamic change law of carbon and nitrogen is just the opposite; the content of five kinds of Mineral elements was Ca>Mg> Fe>Mn>Cu, and presented different dynamic change in different growing periods.Plant polyphenols and flavonoids are the secondary metabolites of plant and also important allelochemicals. Through preliminary experiment of plants’chemical constitutes, it can be seen that Damascus rose leaves contain plant polyphenols and flavonoids, while the branches contain only plant polyphenols without flavonoids. The results of plant polyphenols content determination show that plant polyphenols content in Damascus roses’leaves was higher than that in branches; plant polyphenols content in non-flowering leaves is higher than that in flowering leaves; plant polyphenols content of flowering and non-flowering branches is basically the same; plant polyphenols content presented zigzag dynamic changes in different growing periods.The results of the investigation about the allelopathy of Damascus roses’leaves and branches water immersion show that rape seeds are most sensitive to water immersion, and have the strongest inhibitory effect; sorghum seeds have good tolerance for water immersion and better resistance; wheat and radish seeds show double concentration effect, namely, when concentration is high, growth is inhibited, and vice verse. Allelopathy of leaf water immersion is obviously higher than that of the stem water immersion, which is in line with the fact that leaves contain more allelochemicals plant polyphenols.The best test condition examined in this paper for determining soluble polysaccharide of rose leaves and branches, protein, micronutrient elements, and the content of plant polyphenols can be used as the basis for determination of similar elements mentioned above in plants of the same kind. The allelopathy and dynamic change of nutriments in rose leaves and branches in the growing seasons provide significant reference for further study of physiological effects of nutrients on rose growth and flowering, cultivation management of Damascus rose and comprehensive utilization of rose resources.