| Crops suffer various abiostress in their growth process, such as low temperature,drought, high salt and other environmental factors, they play important roles in agriculturalproduction. Among of the abiostress, high temperature is a key limiting environmentalstress factor for the production and quality of wheat. Therefore, it is of great significationto improve the thermotolerance of wheat.The aims of this study are to isolate the genes from wheat induced by heat stress, toidentify their function by overexpression in Arobidopsis, to improve wheat heat-resistance.Four different sequences, contained complete ORF, were cloned in silico fromGenBank by BLAST using the four up-regulated probes (Qin et al,2008). Sequenceanalysis showed that the four sequences contain conserved bZIP domain, belong to thebZIP transcription factor superfamily, named TabZIP3, TabZIP4, TabZIP5and TabZIP6respectively.Expression of TabZIP3，TabZIP4and TabZIP5genes were up-regulated in heat treatedwheat by real-time PCR, while TabZIP6were down-regulated. It suggests that they maytake part in heat stress response.In order to investigate the biological function of TabZIP genes, the overexpressionvectors of four TabZIP genes are constructed and transformed into Arabidopisis thalianaby Agrobacterium-mediation. Ten TabZIP3transgenic lines were gained by screening. Inregular growth conditions, the phenotypes of TabZIP3transgenic lines were the same asthat of wild type. After45℃heat treatment, six of the transgenic lines show20%highersurvival rate than wild type, These results suggest that TabZIP3gene contribute to heatresistant of plant. |
