Detection Of Ivermectin Pour-on Blood Drug Level In Cattle

The parasitic disease is currently one of the most common animal diseases in livestock production, causing huge economic losses to livestock production. Avermectin (AVM) and Ivermectin (IVM) have Irreplaceable effect in arthropod of parasite infection and alimentary canal threadworms. AVM and IVM Commonly used in veterinary clinical Mostly oral and injection. oral and injection in the prevention and treatment of cattle, horses and other animals process need for Baoding. a lot of inconvenience to the prevention and treatment of parasitic diseases. Not only oral Inconvenient to use,but also Bioavailability is low. However, due to Achieve The best results of injection need professional skills, So injection have a certain degree restrictions. IVM pour on came into being. Ivermectin pour on is a transdermal absorbent cutaneous permeable agent. The drug through the skin into the blood circulatory system to play the systemic efficacy. The biggest advantages of avoids the hepatic first pass effect.Ivermectin pour on direct into the blood circulation Effective debug the endoparasite and ectoparasite.In order to clarify the ivermectin pour on of pharmacokinetics diversity in cattle. Comparative Study of pharmacokinetic characteristics of Jinhe Biotechnology Co. Ltd. developed the ivermectin pour on,united States Ivermectin Pour on and Ivermectin Injection.Select10Mongolian cattle, Randomly divided into4groups. Total of13blood collection point which venous blood collection at2h to60d.Centrifugal separation of plasma which preservation at-20℃for detection. AVM as internal standard substance was developed for determination of IVM plasma by high-performance liquid chromatography with fluorescent detection. Methanol was applied in extracting IVM and depositing proteins in plasma. The extract was purified and concentrated by ODS C18SPE column. Nitrogen gas to dryness the eluate at50℃. The residue was derived by methylimidazole and triflaoreacetic anhydride. Then the derivatives of IVM and AVM were detection by HPLC system. The mobile Phase of proportion:(methonal:Methyl Cyanides=1:1, v/v)/water3%,at a flow rate of1.0ml/min. The column temperature was27℃.The injection sample volume was20μL. The Fluorescence detector was fixed at an excitation wavelength of365nm and an emission wavelength of470nm.The liquid chromatogram analysis20min. In the described chromatographic conditions, Avermectin and Ivermectin were well resolved with no interference from endogenous compounds and with retention times of6.514min,10.987min, respectively.Comparative Study of pharmacokinetic characteristics and catabolism circumstance of Jinhe.Biotechnology Co. Ltd. developed the ivermectin pour on, united States Ivermectin Pour on and Ivermectin Injection. The main pharmacokinetics parameter:medicamentous peak concentration Cmax(ng/mL); peak time Tmax(d); Area Under Curve AUC(ng/mL*d); absorption half life T1/2ka(h); elimination half life T1/2ke(h) no significant difference, Ivermectin Injection significant difference.The results show that can achieve the effective concentration and duration of debug the gastrointestinal nematode.