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Study On Formation And Carbon Sequestration Of Like-Humic Substances In Different Microorganism

Posted on:2013-05-09Degree:MasterType:Thesis
Country:ChinaCandidate:W N ZhuFull Text:PDF
GTID:2233330395963557Subject:Environmental Engineering
Abstract/Summary:PDF Full Text Request
Carbon element is a biological organic ingredients of all kinds of main elements, the rapid increase of CO2in carbon cycle triggered global warming and a series of environmental problems, which threats seriously to the security and existence of human society, how to effectively fixing carbon becomes one of the problems of the present. In order to research the microbial solid carbon process, whether form humus or kind of humus, this paper use two kinds of bacteria in the soil X1(Brevibacterium sp), X2(Bacillus megaterium), three actinomyces F1(Streptosporangium sp), F2(Streptomyces sp.), F3(Streptomyces prunicolor),3kinds of fungi Z1(Trichoderma viride), Z2(Aspergillus), Z3(Penicillium), in the culture solution of glucose, in view of the bacteria after trained by glucose metabolic products and the form of the bacteria, analyzed the bacteria in the use of carbon metabolism and transformation, the use of the elements, infrared spectrum analysis and other analysis, then other means to research the bacteria and its formation in the kind of humus composition, structure, function the difference, and discusses the bacteria through the glucose matrix whether can cultivate form or kind of humus and stability of the degradation. The experimental results are as follows:(1) Different kind of microorganisms in he culture solution of glucose, the carbon content of metabolites after training bacteria in the culture solution of glucose reduced with cultivating the extension of time, actinomyces changes a little in carbon content, the decomposition use glucose less, fungal and all the strains of the decomposition of glucose effect significantly, can decompose most of the carbon thoroughly into CO2.(2) The PQ of X1increased with the sequence of logarithmic phase、stationary phase、decline phase, and it demonstrate that X1formed FA-Like fistly, FA-Like decomposed and promoted the formation HA-Like with the extension of training time. X2increased gradually in the logarithmic phse and stationary phase,but the PQ of X2greatly reduced in the decline phase, it demonstrate that X2formed FA-Like fistly, the HA-Like promoted in the stationary phase,the HA-Like decomposed in the decline phase,and the FA-Like increacd.(3) The difference of training period with different bacteria glucose after the bacteria of formation of the FA elements is very big, in the popular X1H/C value is the largest, most of the ethnic fat; X2aliphatic sex are the strongest, followed by the bacteria. In the bacteria and fungus of the FA infrared spectrum analysis, the X1stable of infrared spectra of the FA in peak form is weak; Logarithm period of X2aliphatic together methylene chain peak form not obvious, and the characteristic peak humus are obvious, the decline of the period of FA degradation characteristics enhancement, aromatic sex weakened; The bacteria aliphatic gather methylene chain the highest content, representative amide C=O stretch, and aromatic C=C stretch and aliphatic C-H deformation and replace the aromatic ring stretch a absorption peaks is two kinds of bacteria in the decline of the formation of the period of the peak form FA significantly. Bacterial differential thermal analysis of the FA, the FA and circulating X1of other large difference between the treatment, the high temperature exothermic peak form not obvious, aromatic sexual structure is few, aliphatic sex is strong; Decay period than bacteria, the fragrance of the X1sex is stronger; The bacteria and the X2aliphatic sex is stronger.(4) Bacteria in the logarithmic period and decay of the formation of the period of aliphatic sex of HA little change, period of decline aliphatic sex enhancement, and all the more bacteria were more aliphatic sex. In the bacteria and fungus HA infrared spectra of the analysis, the bacteria in the logarithm of issue of the structure of the HA aliphatic gather methylene chain contents aliphatic gather methylene chain content more, to decay issue of the kind of aliphatic together when HA methylene chain little content; The period of the logarithm HA obvious trait humus, training cycle with weak; Each peak form apparent all bacteria, especially on behalf of aliphatic gather methylene chain, humus characteristics and aromatic sexual peak form. Bacteria and fungi in all the HA differential DTA analysis, the fragrance of the sex of HA culture period with down, all the kind of HA fragrance of bacteria than strong sex bacteria.(5) Different period of Hu production rate of bacteria with training time increase gradually, all the bacteria in the kind of Hu rate is higher than the bacteria. The elements of show that the bacteria of methylene Hu is together for the structure of the average chain high condensation degree material. By IR and differential thermal analysis combined with, in the period of decline X1Hu gather methylene chain most, is in decay of the formation of the period of the most has the characteristics of corrosion Hu colonization, decay of the content of Hu was greatest; X2in logarithmic period and the period of decline Hu gather methylene chain most, logarithmic period of Hu has formed the colonization of corrosion properties, decay of the period of Hu was the biggest content; The bacteria and bacterial decay issue of the kind of Hu gather methylene chain most, amide content is the largest, most weak budding corrosion properties. Differential DTA analysis shows that, the bacteria in the form of circulating Hu fragrance of the strongest sex, followed by the bacteria.
Keywords/Search Tags:different microbial in soil, HS-Like, culturate budding material, structurecharacteristics
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