Study On Diagnostic Methods Of The Trichinellosls By Immune Colloidal Gold Technique

Trichinosis is caused by Trichinella one of the world distributionof zoonotic parasitic diseases. Trichinosis is not only pose a seriousthreat to human health， but also cause huge economic losses to thepig industry， meat processing industry， the export industry.According to data reported， the vast majority of China’s provincesand cities have incidence of trichinosis cases， swine Trichinellahigh infection areas of Henan， Hubei and Yunnan provinces， theinfection rate of13%， the canine Trichinella high infection area isNortheast， the detection rate of20-40%. The prevalence of humantrichinosis at least17Chinese provinces， municipalities andautonomous regions， including the individual regional humanTrichinella detection rate of2.3%. People suffering from trichinosis，if not timely diagnosis and treatment， the mortality rate of up to3-30%. Low-infected areas of the the province originally swineTrichinella spiralis， after the1990s， with the development ofmarket economy， the more frequent inter-regional flows of animalsand meat without quarantine， cause trichinosis spread ofpathogens， its popular range of further expanded in some areas of the province in recent years have swine trichinosis occur. At present，the domestic swine trichinosis quarantine is still using visualinspection and microscopic examination of the tablet-based detectionrate of only50percent， many of China’s exports of pork testedTrichinella be returned to me The hair of the economy has causedserious losses. Therefore， the establishment of specificity， highsensitivity， and fast for pig slaughter and quarantine swinetrichinosis diagnostic methods， the actual production is very urgentand necessary.The specificity of the test’s application of a high sensitivity，simple， fast， low-cost， easy field application of immunologicaldiagnostic methods. Purified using affinity chromatography or othertechniques the antigen， create a colloidal gold rapid diagnosticmethod. The collected worms first of all swine Trichinella throughthe mouse preserving passaged prepared crude antigen， cultured invitro preparation excretion-secretion antigen analysis of the twoantigens， ES antigen specificity and sensitivity than crude antigen，select ES antigen-based diagnostic antigen test to improve thediagnostic specificity and sensitivity of the method. The principlesof immunochromatography， and colloidal gold labeling techniquewas then applied to the diagnosis application trisodium citratereduction method， gold chloride acid solution is reduced to colloidal gold particles， and in the weak base under the conditions，and Trichinella spiralis ES antigen binding， forming a stable Theprotein markers developed swine trichinosis diagnostic test strip.Trichinella positive serum in38pigs，33pig roundworm，21pigsnarrow neck cysticerci，26cysticercosis positive serum and98points swine Trichinella negative serum were detected and comparedwith ELISA test simultaneously repeated trials， the results of thetest strip to a sensitivity of97.38%，98.88%specificity， andreproducibility of100%. The experimental results show that thecolloidal gold diagnostic test strip with good sensitivity， specificityand stability. Trichinella whole blood and serum can be detected twokinds of different samples. In future clinical application siteslaughter inspection swine trichinosis provides a simple， accurateand rapid test method.The test strip through many trials proved to be able to diagnoseswine trichinosis， and has high sensitivity， specificity，reproducibility， short reaction time， the diagnosis of highefficiency， low cost， easy to operate， easy to determine theresults， easy application， is an effective means for the currentdiagnosis of swine trichinosis.