Molecular Epidemiology Investigation Of Porcine Reproductive And Respiratory Sydrome In Changchun And Its Surrounding Regions

PRRS is a highly contagious disease, mainly caused by the reducedsow reproductive failure and sow life, as well as respiratory diseasescaused nursery pigs; immunosuppression in any day-old pigs caused morepronemixed infection and secondary infection, aggravation of the clinicalsymptoms of the pigs.From the discovery of the22years, the farmscaused enormous losses, PRRS infection is endemic region in the world,China is also not spared. The outbreak of highly pathogenic blue-eardisease in many southern provinces of China in2006, resulting in thedeath of large quantities, please circle a result, many farms, economiclosses, after some domestic research institutes confirmed as of PRRSVNSP2caused by genetic variation, since the occurrence of highlypathogenic blue ear disease since the country has reported thatwidespread in most of our farms, but the incidence rate is different. JilinProvince, most parts of the frequent occurrence, resulting in a great loss,and understanding of epidemic law and the variability of the virus in theregion PRRS cyclical situation of Changchun City and the surroundingarea from June2009to December2010193head suspected of PRRSinfection by App pathogen detection, prevention, and prevention andcontrol of PRRS provide strong experimental basis.In this paper, the RT-PCR method to sample detected193suspectedof PRRS infection by App classical strains amplified fragment of274bp,while the highly pathogenic PRRSV amplified fragment of187bp changeprimers can distinguish between good classic PRRSVthe highly pathogenic PRRSV RT-PCR diagnostic methods, saving valuable time.The number of positive test results of the RT-PCR method, the positiverate is48.7%. HP-PRRS infection80samples, the positive rate was41.45%, the infected classic PRRS14, the positive rate was7.25%; pointof view from this18-month investigation, the incidence is decreased, maybe related to the vaccine positive immune-related.By selected regional representative strains positive for HP-PRRSVNSP2gene cloning, sequencing, and analysis of homology betweenregional strains, popular at home and abroad strains between homologythe intrinsic relationship between the Changchun City and thesurrounding areas at home and abroad strain by sequence analysis toidentify the variation characteristics of the strain, provide strong evidencefor clinical medicine, Changchun City and the surrounding area farms pigpurification.