SSR Variations Of Wheat-rye Derivative

Rye (Secale cereale L,2n=2x=14, genome RR) is an important allied plants for improving common wheat (Triticum aestivum L.,2n=6x=42, genome AABBDD). In a previous study, we obtained30Fg lines with42chromosomes, derived from a interspecific hybrid plant between common wheat D-2-3-4and rye cv. Qinling. They do not contain rye chromosomes. In theory, they should have same genetic consititute. In order to test this inferrence, we compare the DNA consititutes of the30lines by using44microsatellite (SSR) markers. This kind of work is helpful to understand the production of genetic variation induced by wide hybridization and to provide genetic variation for wheat improvement. The results are following:1. Among the44markers,19lines revealed variations among the30lines, range from3.33to14.29%. The rest markers, however, amplified bands in all the30lines were as same as those amplified from parental plants.2. Compared to wheat parent D-2-3-4,14lines (ZBY34, ZBY39, ZBY43, ZBY57, ZBY63, ZBY65, ZBY66, ZBY68, ZBY70, ZBY71, ZBY72, ZBY73, ZBY74, and ZBY76) out of the30lines showed variations ranging from2.27to26.19%.3. Markers Xwmc361(2B), Xwmc168(7A), Xcfd35(3D), Xcfd72(1D), Xwmc511(4B) were used to amplify PCR products from lines of ZBY34, ZBY39, ZBY43, ZBY63, ZBY66, ZBY68, ZBY74. Bands with different sizes from their wheat parent D-2-3-4were recovered, cloned into PMD-18, and then sequenced. The results indicated that the sequence variations were caused by loss or gain of repeated units.