| Rice is one of the most important food crops in world of which yield occupied over half of the total yield of grain in our country. As China was a typical poor-water country,Water resource distributed unevenly both in period and space, which had immensely limited the rice production and development. Taking research of drought tolerance in rice is very important to water-saving irrigation. With the development of molecular markers and the accomplishment of rice genome sequencing, the scholars around the world identified lots of QTLs of yield, quality, insect resistance and disease resistance of QTLs..In this study, F2:3population derived from the cross between non glutinous rice kongyul31and dry rice xiaobaojingzi were used as the experimental materials to construct the genetic linkage map and detectQTLs controlled the sword leaves traits and yield traits under drought and common condition,in order to provide the theoretical foundation for non glutinous rice drought tolerance breeding and molecular marker assisted selection.The main results were as follows:1. A molecular linkage map was constructed based on99SSR markers.This linkage map was a total of1238.42cM in length with an average marker interval of12.51cM.2. All examined traits of F3population under two cultivating conditions followed a normal distribution. Transgressive inheritance of F3population was obvious.3.Plant height, tillers, effective ears, sword leaves length, and sword leaves width were highly significantly and positively correlated with ears weight under drought stress; Plant height, tillers, effective ears, sword leaves length, sword leaves area and ears weight were very significant positive correlation under common condition; Sword leaves width and ears weight were significant positive correlation; sword leaves basal angle, sword leaves stretch angle and ears weight were very significant negative correlation.The results showed that with the reducing of plant height, tillers, effective ears, sword leaves length, sword leaves width, sword leaves area, the production decreased under both of the two cultivating conditions.4.Using the composite interval mapping method, we identified QTLs for plant height, tillers, effective ears, ears weight, sword leaves length, sword leaves width, sword leaves area, sword leaves basal angle and sword leaves stretch angle under two cultivating conditions. The results were as follows:(1)Six QTLs for PH were detected. Of them, two were located on chromosome5、7under drought stress, four were located on chromosome3、6、7under common condition. (2)Two QTLs for TI were located on chromosome2、4under drought stress.(3)Four QTLs for EE were detected, three of them were located on chromosome3、4、6under drought stress, one was located on chromosome2under common condition.(4)Five QTLs for EW were detected. Two of them were located on chromosome5、7under drought stress, three were located on chromosome3、6、7under common condition.(5)Seven QTLs for SLL were detected. Four of them were located on chromosome4、5、7、12under drought stress, three were located on chromosome4、5、7under common condition.(6)Eight QTLs for SLW were detected. Four were located on chromosome3、4、5、9under drought stress, four were located on chromosome3、4、5、9under common condition.(7)Five QTLs for SLA were detected. Three were located on chromosome4、5、7under drought stress, two were located on chromosome4、5under common condition.(8)Six QTLs for SLBA were detected. Three were located on chromosome4、7under drought stress, two were located on chromosome1、7under common condition.(9)Four QTLs for SLSA were detected. Two were located on chromosome4、7under drought stress, two were located on chromosome1、7under common condition.(10) Four QTLs of relative traits controlling drought tolerance were detected. One QTL for relative EE was detected on chromosome2. Two QTLs were detected for relative EW on chromosome6and12. One QTL was detected for relative SLSA on chromosome11. |
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