Construction Of RIL Population And Preliminary Study Of SSR Linkage Maps Based On Sequenced Rice Varieties

Rice is one of the major crop for human consumption, providing staple food for more than half of world's population. Most important agronomic traits of rice are quantitative, such as yield, quality and stress resistance and so on, which are controlled by polygene. so It's very important for rice breeding improvement to map quantitative trait loci (QTLs) accurately,, Specially, it is very useful to clone QTL using the materials which genomic full-sequence has been known, in this study, using the combination of Pei'ai64S/Nipponbare for constructing RIL population, evaluated RIL population based on phenotype value and genotype value, we constructed rice SSR linkage map based on RIL population, compared it with the F₂ map from the same cross Pei'ai64S/Nipponbare and the sequence map of Nipponbare, respectively ? we analyzed the segregation markers, then 3 types of agronomic QTL were mapped on the map The main results were as the following:1.Using the F₁ descendants of combination Pei'ai64s/Nipponbare for constructing RIL population, F₂ F₄ F₆ were planted in wen jiang county Sichuan province and F₁ F₃ F₅ were planted in lingshui county hainan province, respectively The purity degree of 3 types traits "tiller number panicle number plant height" is 60.61% 90.0% 50.61%, respectively The purity degree of RIL population with molecular marker is 96.03%,mixed degree is 3.97%, respectively 2.Examined polymorphisms of bi-parent by 515 primers, there were157 primers Showed polymorphisms, the ration of polymorphisms was 30.48%, we used these Markers for constructing a linkage map of 92 markers, including 17 linkage groups, the total genetic distance of the map was 2563.5cM, the average distance of two adjacent markers was 27.86cM ,3.compared with F₂ map, there were great difference about linkage number of markers marker order total genomic length average marker distance on two maps ; compared with G-map, of the 92 markers, 74 were tagged to G-map, others 18 markers were not located on the same chromosomes on G-map, 74 SSR markers, covering 159.8 Mb of genomic length with an average interval size of F₂map 2159.46kb4. There were many significant segregation distortion markers in RIL population, 63 markers(68.48%)were distorted on the F₆map, of 63 markers(P<0.05), 60 markers deviated toward Pei'ai64S, only 3 markers were deviated toward Nipponbare; there were others 65 markers without mapping, 27 markers were distorted 38 marker were segregated normally 5.60markers(95.24%)showed deviation on the map were mapped chromosomesl 2 3 4 5 6 8 9 11 12, respectively, most markers were in segregation distortion regions, named as followed: SDR1-1 SDR1-2 SDR2 SDR3-1 SDR3-2 SDR4, SDR5-1 SDR5-2, SDR6-1 SDR6-2, SDR8 SDR9-1 SDR9-2, SDR11 å’Œ SDR12, Most of SDR deviated toward Pei'ai64S, only two markers of SDR1-1 deviated toward Nipponbare,6.There are total 3 QTLs identified with interval mapping, Two QTLs Controlled tiller number and another one Controlled panicle number, No QTLs was identified about plant height...