A Preliminary Study On The Change Rule Of Reproductive Hormone During Different Physiological Period Of Female Buffaloes

These studies were conducted to investigate the change rule of five principal reproduction hormones,such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), inhibin (INH), estradiol (E2),and progesterone (P4) during different physiological periods of female buffaloes by the enzyme-linked immunosorbent assay(ELISA) and B-mode ultrasonography. Results were sumrized in following:(1) Different varieties of female buffaloes have the similar periodic change rules of the E2and P4concentrations of the serum during the low breeding season and the breeding season,but there were different level of E2and P4between the season and among the varieties.(2)The concentrations of the E2and P4have correlation with the oestrous cycle, the high concentrations corresponding the short oestrous cycle, vice versa.(3) The level of FSH and LH was highly significant positive correlation(P<0.01), the same as INH and E2,(P<0.05); the FSH and INH was highly native correlation(P<0.01), the same as INH and LH(P<0.01), INH and P4(P<0.05), E2and P4(P<0.01).(4)Although the P4levels of the female buffaloes have some changes during early pregnancy, they were maintained in a higher level (above20ng/ml) than postpartum (5to7ng/ml).(5)The P4concentrations of the most female buffaloes were declined to the base level within15days of postpartum, then the level was rised after23days accompany with ovaltion and corpus luteum formation.(6)The concentration of E2have declined to the base level of the female buffaloes within11-14days of postpartum, and rised in18-20days, accompany with follicle development.In conclusions:the principal reproductive hormones in different breeds display similar changes rules varieties during the different breeding season and early pregnancy and postpartum, but their concentration is different in different season and different breeds.