Isolated Microspore Culture And Genetic Analysis Of DH Population In Pak Choi

The objective of this paper was to study the optional conditions for isolated microspore culture for the Pak choi and the heritability analysis of seven agronomic characters in non-heading Chinese cabbage by using the method of identifying mixed major genes and poly genes. The results were as follows:Several key factors affecting isolated microspore culture in Pak choi(Brassica campestris ssp.chinensis Makino var.communis Tesn et Lee) were studied by using three gene types of it. The mono-factor and multi-factor experimental results indicated that gene type and vernalization played an important role in the microspore embryogenesis. The presence and concentration of sucrose and activated charcoal had great influence on the frequency of microspore-induced embryos. Moreover,2days of low temperature treatment,0.2g/L activated charcoal and24h of high temperature treatment were of benefit to embryos induction.With a double haploid (DH) population derived from F1which cross between NJ089and NJ074, the heritability analysis of seven agronomic characters in Pak choi (Brassica campestris ssp.chinensis Makino var.communis Tesn et Lee) was conducted by using the method of identifying mixed major genes and polygenes. All examined characters showed the models of two major genes plus polygenes except that bolting index showed a model of three major genes without polygene. The list of heritability of major genes from high to low was leaf color, plant spreading, the largest of leaf width, the largest of leaf length, chilling injury index, plant height.