Study On Induction Factors Of Embryogenesis Of Isolated Microspore Culture Of Mustard

The relation of mononuclear microspores step aside, bud size and the length ratio of the petal and the anther was investigated in four genotypes of leaf mustard, in order to determine the appropriate microspore culture of bud length. Meanwhile, NLN-13 is used as basic culture medium. By cultivating 60 genetic types of leaf mustard on isolated microspore culture, we selected 11 genotypes with Embryogenesis, then chose one of several genotypes, culture medium were treated with different concentrations sucrose, activated carbon, according to different process, such as colchicine treatment, tested materials at low temperature pretreatment, microspore heat shock treatment and handling of microspore culture density, we can research on the impact of isolated microspore culture embryogenesis to study the factors, the results are as follows:1. Bud, petal and anther length ratio of leaf mustard were closely related to the developmental stages of microspore. We can use what I mentioned above to be a indicators, judging the Microspore population suitable for inducted cultivation or not. For leaf mustard microspore embryogenesis in microspore development stage of the single-core step aside, step aside a single core share of more than 50% of it is good, the corresponding bud length 2.5-3.0mm, the length ratio of the petal and the anther 2/3.2. Using NLN-13 medium,60 leaf mustard genotypes were isolated microspore culture, and selected 11 genotypes with Embryogenesis, the embryo ratio of 18%, the materials in the embryo, the embryo varies widely, of which the embryo QQ-2 the highest rate of 5.84 embryos/bud,3A-22-84 to 5 embryos/bud, QQ-1 was 3.75 embryos/bud, YQqc-1 to 2 embryos/bud,3C-22-5 to 1 embryos/ bud, DSqg 0.87 embryos/bud, DSqg 0.75 embryos/bud, ZXzh 0.62 embryos/bud, YQ-1 was 0.37 embryos/bud, Qnong-4 was 0.25 embryos/bud, DQky the embryos was the lowest,0.12 embryos/bud, results indicate that the genotype is major factor, which affect small spores embryogenesis of leaf mustard.3.4℃low temperature was used to 10cm long inflorescences of leaf mustard, which have positive effects on the small spores development. If the treatment time of low temperature less than 4d,we'd better use 1d bud to deal with.4. Activated carbon was used as 0.3g/L and 0.5 g/L in the medium to the leaf mustard Embryogenesis, we can find out that the development leaf mustard Embryogenesis was stimulated, and the culture effect is best with activated carbon 0.5 g/L, but microspore embryogenesis can be inhibited by overdose activated carbon.5. It is the most appropriate inoculation density for the leaf mustard to inoculate 8-10 buds in eachΦ60mm culture dish, and 8buds per dish is best.6.30-80 mg/L of the colchicines was used to the medium, it obviously promote the leaf mustard microspore embryogenesis, but the best concentration are different among different genotype.7.The most suitable sucrose concentration of leaf mustard microspore culture was 13%.8. The optimum temperature of leaf mustard heat shock treatment was 33℃, 24h.