The Effect Of Chinese Herbal Formula On Rumen Environment And Diary Cattle Performance

Antibiotic had accelerated the development of feed industry as a sort of additive application in production. But in the recent year, the irrationality application of antibiotic result in the problem residue, resistant seriously increasingly, indeed, deteriment the human’s healthy. Chinese herbal medicine come from the nature, possess great medical value and safety. Take it as feed additive apply in production must obtain some advantage. In the experiment, we focused on enhancing the production performance of the cows in the period of lactation, indeed, keeping the cows healthy. The experiment included three sections:In experiment1, the single outflow continuous culture system was applicated, rumen fluid inoculum for in vitro trials was collected from the15healthy, castrated and ruminally fistulated sheep. Four formulas (A) were designed and3levels (B) respectively, the ratio to concentrate was5%,10%and15%. Each with3repeats, setted control trail.7days for adaption and7days for studying. Indeed, with the factors analysis experiment design, we selected the formula and the dosage to prepare for the following experiment. The results showed that Chinese herbal medicine could improve the rumen environment and the fermentation level. The pH varied from6.91to6.98, stayed in normal range, and the differences were not significant between added groups and control (P>0.05). Comparing with the control group, the gas production of added groups were higher, when the dosage was5%, the formulas2and3were higher than control significantly (P<0.05), when the dosage was10%, formula1,3and4were significantly higher than control (P<0.05), when the dosage was15%, formulas3and4were significantly higher than control (P<0.05). Comparing with the control, NH3-N concentration in all added groups were lower than control when the dosage was5%, but the differences were not significant (P<0.05). When the dosage was10%, the NH3-N concentration of added groups increased, but the differences with control were still not significant. When the dosage get15%, the NH3-N concentration still increased, and the formula1,2and3were significantly higher than control (P<0.05). The BCP concentration in all added groups were higher than control, and when the dosage was5%, formula1,2and3were significantly higher than control (P<0.05). When the addition added to10%, the formula2,3and4were significantly higher than control (P<0.05). However, when the dosage was15%, the BCP concentration in the four formulas were lower than control, the differences were not signicant (P>0.05). With the addition increasing, NDF disappearance rate in all the four formulas improved significantly than the control (P<0.05). The ADF disappearance rate changed in the same trend. Through the MFAEI of varity formulas and levels of Chinese herbal formula effected on the fermentation indexes in vitro, we selected the formula1and formula2with the dosage5%, and the formula3and formula4with the dosage10%to study further.In experiment2, we take15healthy, annulated and castrated sheep as model animal to study the effects of Chinese herbal formula on rumen microorganisms. The sheep were allocated into5groups randomly with the single factor experiment design. The sheep of the four added groups were provided the ration with Chinese herbal formula formula selected in the experiment1, setted control trail. After the14days feed probation, we collected the rumen contents for3days to study the effects of Chinese herbal formula on the number of cellulose-decomposing bacterias. The results of the real-time qPCR showed the order of magnitude of Ruminococcus flavefaciens was6, the number of all the added groups were higher than control, and the result of experiment group Ⅲshowed the largest number, but the differences were not significant (P>0.05). The order of magnitude of Ruminococcus albus number was7, the results of all the added groups were higher than control, the difference between experiment group III and control was significant (P<0.05), the others were not significant (P>0.05). The detected results of Fibrobacter succinogenes with real-time qPCR was that the order of magnitude was8and the number of all the added groups were higher than control, the number of Fibrobacter succinogenes was largest in the experiment group Ⅱ, indeed, different significantly with control (P<0.05). All the real-time qPCR results mentioned in experiment2showed that the Chinese herbal formula had positive effects on the growth performance of the cellulose-decomposing bacteria, and the formula3with dosage5%had apparently effect on the growing of Ruminococcus flavefaciens and Ruminococcus albus, and so did the formula2with the dosage on the growth of Fibrobacter succinogenes.In experiment3,50healthy cows which had the same parity, in the same duration of lactation and the similar body weight were selected to study the effects of Chinese herbal formula on the milk performance and the serum biological indexes. The cows were allocated into5groups randomly and10cows each group respectively.4groups added the Chinese herbal formula selected in the experiment1, the other one was control.15days for adaption and the trying period was35days. The results showed:when the experiment finished, the milk production in all the added groups were significantly higher than control (P<0.05). The butter-fat content in all added groups were higher than control, and the difference was significant between the added group Ⅳ and control (P<0.05). The milk protein content in all added groups were higher than control, but the differences were not significant (P>0.05). The ALP content in serum of added group Ⅰ, Ⅲ and Ⅳ were significantly higher than control (P<0.05). The BUN content of serum in all the added groups were higher than control, but the differences were not significant (.P>0.05). The MDA content in all the added groups were higher than control, and the added group Ⅰ, Ⅱ and Ⅳ were higher than control significantly (P<0.05). The SOD content of serum in all the added groups were higher than control, the differences were not significant (P>0.05). The T-AOC activity in serum of the added group Ⅰ, Ⅱ were higher than control significantly (P<0.05), and the added group Ⅲ, Ⅳ were higher than control but the differences were not significant (P>0.05). The IgG and IgM content of serum in all the added groups were higher than control, but the differences were not significant (P>0.05).