The Poria Strains Quality Standards And Germplasm Research

Purification of fine strains, is the fundamental guarantee of success poria cocos cultivation, the direct impact of the product quality and cultivation has production, then influenced growers economic benefits. In recent years, the research of strains insufficient investment, have lagged behind in production situation. Because the lag of breeding, the country was introduced to each other alternative new varieties, in the name of product, the business name, a scientific name and the confusion and confusedly frequently occurred, linebreed name phenomenon more optional names Numbers is more common, as from the introduction of the different strains, random Numbers, to cover up the real source and Numbers caused chaos strains.At the same time, speed up including proteins and DNA fingerprints, poria cocos germplasm resources with more information database realistic significance.This topic at all levels according to the strains of preparation and poria cocos growth conditions at all levels to determine the quality standard strains tuckahoe. To determine the screening of the culture medium strains tuckahoe has the most suitable of growth medium. At the same time, the esterase technology and ISSR molecular markers of our country, and some wild strains of the cultivation poria cocos strains of germplasm characteristics and genetic diversity is analyzed. The main research results are as follows: First:The Poria strains quality standards and germplasm researchThrough the antagonist experiments show that, some of these strains performanced obvious antagonistic phenomenon. For example, Ma cheng Jing28,P0--ACCC50864,etc,indicating their genetic system differences.Some strains antagonistic phenomenon is not obvious, forexample:P0-Jing28,indicating that small genetic differences may be of a synonym.Observed Poria mycelial growth of the Poria cocos to determine the growth rate and germination of the colonization ability:in the PDA medium, in the optimal temperature (24℃±℃), germination engraftment time for1-2d, covered with a tube of time for cant5-8d. In the pine wood shavings77%, wheat or rice bran20%, sugar2%, gypsum1%of the culture medium,25to30℃temperature, the hyphae of bacteria bag for15to30d commonly.Second:The Poria strains screening studies of the mediumPoria strain medium filter,including the comparison of solid and liquid medium,and discover the most suitable for the solid medium for common culture medium, liquid medium for maltose medium.Third:The Poria strains esterased isozyme researchPoria strains taken from different sources30esterase isozyme analysis of isolates tested were detected in211of esterase isozyme bands of the enzyme in each strain ranging from6-11bar.The majority of six.30strains tested a total of14kinds of zymogram types,some of the same strain bands.Clustering analysis shows that:when the genetic similarity level of80%for the test30the Poria strains can be divided into eight categories.The first kind of:E1, Fujian006; The second type:Macheng, ZJ, Tongrentang, Z(Z),50864, P0,86. Jing28、F6、A10、Dabieshan、9、10、14、Y1;The third class:5.78;The fourth class:Poria cocos28; The fifth class:L; The sixth class:YN、Poria cocos number5、W、S1、50876、DB; The seventh class:GD、901、GZ;The eighth kind:ZL.Part of the similarity coefficient is1strains,shows that they close relationships.Fourth:ISSR molecular marker in poria cocos genetic diversity of applicationThe source of the three different strains poria cocos in30ISSR primer for screening, select amplification stable,the10polymorphic primer used for ISSR analysis. In screening annealing temperature, and on the basis of article in10to30poria cocos primers on strains ISSR fingerprint analysis,10primer amplification of the article110DNA belt, which is with article70polymorphism, with64%of the total number, the average primer amplification bands that seven. Clustering analysis shows that, in86%of similar level,30strains can be divided into six categories. The first kind of:Z (Z)、El、Macheng、GD、poria cocos28. Tongrentang; The second type:ZJ、 YN、50876、F6、901、A10、DB、50864、S1; Third class:Poria cocos5;The fourth class:Dabieshan、ZL、86、GZ、Fujian006、Jing28、W、L、Y1、9、P0; The fifth class:10; The sixth class:14,5.78.