The Biological Flinction Analysis Of PvFT1Gene In Phyllostachys Violascens

In this study, PvFT1gene in Phyllostachys violascens was successfully transfered into rice (Oryza sativa), and the transgenic rice flowered in vitro. PvFT1protein was observed in the nucleus of onion epidermal cells through gene gun bombardment. The constructed plasmid pET28a-PvFT1protein was transformed into E.coli and PvFT1protein expressed as inclusion bodies. There were at least two copies of PvFT1gene and each copy drived different promoters. The main results of this paper are as following:1. CaMV35S:: PvFT1was successfully transfered into rice (Oryza sativa). The differentiation of transgenic callus was affected and the in vitro plantlets were hard to root. Some transgenic seedlings flowered even in vitro and died after flowering.2. The recombinant plasmid pCHF3-PvFT1was transferred into onion epidermal by gene gun bombardment, and PvFT1protein was detected in the nucleus by observing fluorescence microscopy.3. The recombinant plasmid pET28a-PvFT1was transformed into E. coli.(DE3). The SDS-PAGE analysis showed that:the molecular weight of the induced protein was about20kDa, approximate to that of the predicted protein, but the expression of PvFT1varied under different temperature and different induction time.4. Two promoter sequences were obtained and the sequence analysis revealed that there was1061bp missing in the short promoter comparing with the long promoter (proL). There were at least two copies of PvFT1genes, which were named as PvFT1a and PvFT1b, and each copy drived different promoters.5. Different promoter fragments were amplified by specific primers designed according to the promoter of PvFT1a, and the expression vectors were constructed of different missing elements of the PvFT1gene promoter. The constructed plasmids were transformed into Agrobacterium tumefaciens GV3101and Arabidopsis were transformed.