Studies On The Effect Of Cultivate Formula, Cu²⁺ And Mn²⁺on The Growth And Development Of Pleutotus Nerbrodensis

Pleutotus nerbrodensis is a rare mushroom whose edible and medical value are very high. It is rich in the physiological activators,such as fungi polysaccharide and vitamin and the minerals. It could balance the body physiology, and strengthen the immunologice function. Due to the increasing price of cultivated raw materials, which increased the production cost, and low biological efficiency of P. nerbrodensis, obstructed further expansion and promotion of its industrialized production. The study screened a new substrate formula in cultivation for P. nebrodensis, studied the effect of mineral elements Cu and Mn on growth and development of P. nerbrodensis and measured exocellular enzyme activity and ion current changes(non-invasive micro-test technique,NMT technique) in different growth stage of P. nerbrodensis. The main results were as follows:1. Studies on new substrate formula in cultivation for P. nebrodensis. The results showed that the peanut shell, cotton stalk, chestnut sawdust could be used as mainly cultivated substrate of P. nebrodensis and replaced the cotton seed hull in cultivation medium partly. The cost-income ratios of all formulas were higher than that of CK. The formulas (Group C)of adding could dramatically reduce the production cost and increase the efficiency of the producers. The optimum formula was C1(40%contton seed hull,40%cotton stalk,15%wheat bran,2%corn flour,2%lime,1%gesso), which yield the per-unit, biological efficiency and production cycle were188.38g,53.81%,150.7d, respectively.2. Studies on the effect of different Mn concentration on the growth and development of P. nerbrodensis. The results showed that the optimum Mn addition concentration was60mg/Kg, of this concentration, the mycelial growth rate, cultivation cycle and yield were3.87mm/d,157.21d,234.70g, respectively.3. Studies on the effect of different Cu concentration on the growth of P. nerbrodensi. The result showed that the optimum Cu addition concentration was50mg/Kg. Of this concentration, the mycelial growth rate, cultivation cycle andyield were4.35mm/d,166.22d,177.30g, respectively.4. Studies on the effect of different combination of Mn and Cu concentration on different cultivated medium on the growth of P. nerbrodensis. The results showed that the growth of the hyphae growing in the formulas of adding100mg/Kg Cu (D5/D6/D7) was obviously superior to that growing in the formulas of adding50mg/Kg Cu (D2/D3/D4) in Group D. With the increasing Mn concentration, the hyphae growth decreased. The formula with highest yield was the D3(170.43g). In group B, the optimum formula was B3which the mycelial growth, cultivation cycle and yield were5.57mm/d,157.82d,196.51g, respectively. In group H, the optimum formula was H6which the mycelial growth, cultivation cycle andyield were4.83mm/d,157.82d,196.51g, respectively.5. Studies on the variations of exocellular enzyme activity in different growth stage of P. nerbrodensis. The results showed that the variation trend of Laccse (Lac) was gradually decreased that it was highest in mycelial growing stage than that in the period of postripeness, temperature stimulation, fruit body growing. The Lac enzyme activity fell sharply after fruiting body harvest. While the variation trend of MnP activity was a kind of parabola that it fluctuated a little between the period of mycelial growth and postripeness, significantly increased in the period of temperature stimulation and reached the highest level in the early period of budding, then gradually declined in the later period of fruit body growth and dropped to the similar activity level at mycelial growth period after harvest.