Effects Of Microelement Manganese And Copper On Physiological Characteristics Of Pleurotus Eryngii

Plourotus eryngii is one kind of precious edible mushroom,The increasingpopularity of P. eryngii among consumers is due to its flavor, texture and shelf life.In this study, three strains of P.eryngii, including Xingbaogu1, Chuanxingbaogu2and Chuanxuan1, were used to demonstrate the effects of microelement manganeseand copper on the ligninolytic enzyme system-manganese peroxidase and laccase byadded to the imiting substrate liquid medium. Manganese peroxidases activity wassignificantly higher when manganese was added to the medium at the concentration10μM～500μM. The peak value of manganese peroxidase was occurred at day23.And the highest value was occurred when MnSO4was added at the concentration of50μM,50μM and100μM.When CuSO4was added at the concentration of10μMand50μM, laccase activity was significantly higher, the highest value were occurredat the concentration of50μM. laccase activity decreases when CuSO4was added atthe concentration of100μM and500μM.Semi-quantitative RT-PCR method was used to study gene expression of P.eryngii strain Chuanxuan1manganese peroxidases and laccase, by adding MnSO4and CuSO4to the imiting substrate liquid medium at the concentration of50μM.firstly, Two manganese peroxidase gene and three laccase gene fragments were clonedfrom Pleurotus eryngii mycelium, and then Semi-quantitative RT-PCR method wasused to study the five genes expression by usingβ-actin gene as an internal gene.Manganese peroxidases gene MP was strongly induced by MnSO4at theconcentration of50μM, laccase gene lac2as strongly induced by CuSO4at theconcentration of50μM, while the other manganese peroxidases and laccase gene hasno significant change.The effects of manganese and copper on the mycelial growth of Pleurotuseryngii were demonstrated. Microelement manganese and copper influence themycelial growth of P.eryngii. Mycelial growth were significantly higher from PDA culture containing manganese at concentration of10μM～1mM of the three stains.While when add copper to PDA culture, Mycelial growth were higher at concentrationof10μM,50μM and100μM,the mycelial growth was inhibited when concentrationof copper become higher.Experiments were performed to determine effects of supplementation ofcottonseed hull substrate with manganese, copper on the yield of P. eryngii. The yieldwere improved when MnSO4is added to substrate at the concentration of10,50,100,200and500mg/kg, and the optimum concentration is50mg/kg, increased the yield ofthe three stains by16.9%,15.4%and14.2%. The yield of strain xingbaogu1andChuanxingbaogu2was decreased when CuSO4is added to the substrate at all of thefive concentrations. While the yield of strain Chuanxuan1were higher when10and50mg/kg copper are added to the substrate.