Watermelon fusarium wilt (Fusarium oxysporum f.sp.nivum) is a soil-borne diseaseprevalent in the world on watermelon production, the mycelium of the pathogen Fusariumoxysporum, chlamydospores spores, sporangia survive over winter in the soil10years,the lifeforce of a strong,difficult to control.Continuous cropping plots with increasing planting years,the pathogenic bacteria in the soil year after year accumulation cause by continuous croppingof watermelon block,with the occurrence of planting the increase of years watermelon wilteven more serious. In rencent years, Fusarium Wilt of watermelon research,people will lookto invest inenvironmentally control, and actinomycetes as an important biocontrol strainresources of concern. This test dish antagonistic activity of Fusarium disease pathogenFusarium oxysporum.Best actinomycetes Act1,been to the actinomycetes Act1agents obtained by ferment-ation, seedling and field tests to study the even watermelon wilt disease control effect of test.Actinomycetes Act1preparation do in the prevention and treatment of the watermelon wiltprovide a theoretical basis to be used to guide production in order to get better controlefficacy.Watermelon wilt pathogen of watermelon seeding inoculation and disease preventioneffect by actinomycetes Act1preparations studies, obtained in the test inoculated with path-ogen concentration:2.5ml,5.0ml,7.5ml of watermelon seedlings have strong pathogenicity toprove the strong pathogenicity of the pathogen; seeding stage application of differentconcentrations (0.5g/plant,1.0g/plant,1.5g/plant)actinomycetes bacteria Act1preparations were inoculated with different concentrations(2.5ml,5.0ml,7.5ml) pathogens, the test resultsshows that has a preventive effect the actinomycetes Act1agents on the occurrence ofwatermelon fusarium wilt, mid-term application of the actinomycetes Act1preparationconcentration was1.0g/plant,1.5g/plant line handling, anti-most-efficient good, respectively80.7%,82.2%.Drawn, with the watermelon cultivation plots continuous cropping to increase theincidence of Fusarium disease is increasing year by year, and even plots of land for threeyears of continuous cropping plots on the different watermelon wilt incidence and investigation of soil microthe incidence rate of29.5%;plots of soil microbial content ofdifferent watermelon planting year ingeneral:bacteria>actinomycetes>fungi,fungi concentpolts of different planting years as follows:3>2>1year block,actinomycetes,bacteria levelsare:1>2>3year block; different watermelon even for plots of soil urease,phosphatase, catalaseactivity in the overall performance of:1>2>3-year block.Different application put line bacteria Act1preparations (nursery application of actinomactinomycetes bacteria Act1preparation1g/plants, planting application of actinomycetesbacteria Act1preparation1g/strain blank control)three treatments on the even for three year,the watermelon wilt disease of anti-disease effects. The results showed that:With years of continuous cropping increased with increasing years of continuous cropping,even for years of land watermelon wilt incidence of aggravated with the increase in thenumber of years of planting.Nursery application of actinomycetes Act1preparation of1g/plant, planting application of the actinomycetes Act1preparation1g/strains on the water-melon wilt have some control effect,the control effect are as follow:51.15%,49.37%;Different content of watermelon rhizosphere microorganisms, bacteria>actinomycetes>fungi, actinomycetes, bacteria content performance as follows:nursery application acttinomycetthe Act1preparations to deal with>colonization application of actinomycetes Act1pre-paration>control (CK),the content of the fungi: control(CK)>nursery application of Act1preparations to deal with>planting application of actinomycetes Act1preparation processing;With years of continuous cropping increased with increasing years of continuouscropping,soil signidicantly reduce the content of the bacteria, fungi, fungal content of thebacteria,fungi,fungal content of a substantial increase in.Nursery and planting inoculated putline bacteria Act1agents can significantly with number of increase in watermelon root zonebacteria and actinomycetes of reducing the number of rhizophere fungi and significantlyimprove the plant root,the occasion of A/F,A/B,B/F improve the health status of thewatermelon plant rhizosphere microbial ecosystem, reduces the occurrence of continuouscropping plotswatermelon wilt;Inoculation of seedlings and colonization changed the enzyme ctivity of soil actinomy-cetes Act1preparation to improve soil urease, phosphatase activity compared with thecontrol; soil catalaseactivity with the control compared to a steady slowtrends;With years of continuous cropping to increase the quality of watermelon decreased. Havesignificant effect the actinomycetes Act1agents on the watermelon severalnutrients. Inoculated with actinomycetes Act1preparation,seedling tray significantly improve-ed watermelon fruit in the Vc, soluble protein and soluble solid shape content. With the increase in continuous cropping of watermelon production year.The fieldapplication of actinomycetes Act1preparations can improve the yield of watermelon. Nurseryplate inoculation treatments yield than planting pit with processing. |