| With the problem of global climate increasingly serious and fossil energy consumption is an increasingly serious, the use of bio-energy has become a trend in the human and social development. Biodiesel as a renewable energy development, utilization has attracted the world attention. High photosynthetic efficiency of microalgae has a large amount of biological growth cycle is short, high oil content, environmental adaptability, easy large-scale cultivation of the advantages of the new biomass energy, new materials for biodiesel production. Hainan is located in the tropics, a tropical monsoon climate, the vast water resources, the annual warmed, abundant rainfall, the algae is so much variety, climatic conditions suitable for algal growth. However,, Hainan research algae research team is relatively weak, limited to the optimization of the algae culture conditions, we can say the algae species of Hainan has just started, there is a great use of space, with the potential research value. In this study, samples were separated, through acquisition Haikou coastal waters and the water of the Hainan University campus screening, optimization, and collected some of the Hainan Te some algae species, has greatly enriched our algae species, to establish a set of algae culture conditions optimization fast, convenient device and filter out through the control of nutrients and nutritional changes in the way with high biomass and oil content algae strains provide a foundation for the development of microalgae biodieselIn this paper through the use of a straw separation, water separation, separation method, dilution plate method, antibiotics separation of14sampling points were algae isolates, optimization, screening out easy cultivation, with oil-producing properties of8strains of microalgae by cell morphological identification, preliminary and affirmatory muelleri, Navicula two Chaetoceros species, the number HNC2, HNC5, HNC6, HNC8, HNC9, HNC11, algal strains were studied, preliminary and affirmatory numbers for HNC2, HNC8, HNC11is Chlorella, numbered HNC9algae is NannochloropsisBy using the0.618method of golden section method to determine the number of HNC11and HNC2algae strains of optimum growth temperature, and homemade light temperature gradient incubator is identical to the result obtained by. By using the device with an optimal Chaetoceros Navicula growth the most suitable light and temperature conditions. From10days of growth rate can be seen in the optimum temperature in19-27/C. Navicula the optimum temperature in25-38/DEG C, below or above the range of algae growth condition is poorer, not conducive to the growth of algaeThe number of HNC8、HNC9algae were researched on N and Fe3+on the accumulation of oil cells. The results show that:N lack of conditions to promote the chlorella and micro-algae the accumulation of oil cells, but growth was inhibited. Compared with the control,the lack of Fe3+in the medium inhibited the growth of Chlorella and oil accumulation;lack of Fe3+micro-algae the oil accumulation inhibited;but no significant effect on their growth, Fe3+rich oil treatment on the accumulation of Chlorella has a stimulating effect, but the micro-algae was no significant effect; Fe3+and normal iron-rich conditions,Chlorella,and micro-algae was no significant difference.On the number of HNC2, HNC5, HNC6algae strains in different methods of nutrition treatment observation of cell growth and fat accumulation effect, obtains the HNC5to autotrophic growth best and later oil accumulation is more obvious, and the HNC6and HNC2in mixotrophic culture conditions grow best, but HNC6in heterotrophic culture conditions under the oil accumulation is significantly higher than the autotrophic to hold concurrently raise, HNC2of algal species in3kinds of nutrition, lipid accumulation gap is not obvious... |
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