Study On Changchun Area Potato Spindle Tubers Viriod (PSTVd) Detection

Potato spindle tuber viroid (PSTVd) is a main disease of potato in the world. Detection techniqueis an effective method to control diseases. RT-PCR has the advantage of rapid, sensitive, simple,specificity, etc. It can detect low content RNA in pant tissue and it has widely used in pathogenidentification. Using RT-PCR detect PSTVd can produce virus-free potato, increase potato quality andyield.In this paper，we collected Changchun area18samples include11varieties, including "Chunshu4","Kexin1","Kexin2","Kexin3","Youjin","Fujin","Yanshu4","Dongnong303","Chunshu5","Atlantic" and "Favorite". Based on the homologous nucleotide sequences of PSTVd3pairs of primerswere designed. Using the method of RT-PCR detect PSTVd, and provide technology instruction forChangchun area.The results showed that "Y217-2" and "09-Y217-1" were PSTVd positive, others were negative.Amplified fragment were highly homologous with PSTVd. There was no evidence show that they haverelationship of PSTVd infect with culture date and culture condition...