Study On Transgenic Potato Resistant To PLRV And PSTVd

Potato is one of the most important crop in the world, and also widely planted in the north of China. In potato production process, the viral disease has becomed the main factors that make seed potato degradation and yield reduction, and cause huge losses to the potato production. In recent years, the genetic engineering technique has becomed effective way to develop potato varieties resistant to viruses and viroid.In this research, potato cultivars of52E,79-09,3-105were transformed by Agrobacterium tumefaciens.The potato’s stems and leaves were used as explants. The transgenic plants were obtained and analyzed at molecular level. The main results are as follows:(1)Using potato stems and leaves as explants, the average callus induction rates were77.1%and67.2%respectively, which showed that both stem segments and leaves discs can be used as receptor materials for transformation;(2)Before the transformation, explants were pre-cultured for2d which increased the transformation rate;(3) Longer Co-culture time of explants and Agrobacterium leaded to overgrowth of Agrobacterium and optimal co-culture time was3days;(4) PCR detection of the obtained transgenic potato showed the target genes were integrated into the potato genome. Northern Blot analysis showed that ribozyme targeting against negative strand of potato spindle tuber viroid (PSTVd) was expressed at RNA level. Transgenic plants were inoculated with PSTVd and RT-PCR detection showed that some transgenic plants were resistant to PSTVd.