Study On Protective Effect And Anti-oxidation Of Blueberries Anthocyanins On CCl4Induced Liver Injury In Mice

Anthocyanins are a group of widespread natural phenolic compounds in plants. They are mainly distributed among flowers, fruits (particularly in berries), and vegetables, and they are responsible for their bright colors, such as orange, red, and blue. Oxidative stress is the result of an increase in intracellular pro-oxidant species such as hydrogen peroxide, hydroxyl radicals, and superoxide anion radicals. The main results are as follows.(1)By separating and purifying the blueberries anthocyanins using macroporous resins, NKA-9is the ideal adsorbing material, which shows good adsorption capacity and desorption ability. The best parameters of static adsorption are40℃,pH4.5, while the NKA-9resin has the largest adsorption quantity of anthocyanins in crude extraction liquid. The best desorption condition is using70%ethanol at30℃, while the desorption rate is the largest, indicates the best desorption effect.(2) In animal experiment, compared with the liver injury model group, in each anthocyanin-dose group and in the control group, SOD and CAT activities in the red cell membrane were significantly increased. Compared with the low-dose anthocyanin group, the middle-and high-dose anthocyanin groups showed significantly increased red blood cell SOD and CAT activities, with a significant difference between the high-dose anthocyanin group and the control group (P<0.01).Compared with the liver injury model group, plasma GSH-Px activity in each anthocyanin-dose group and the control group was significantly increased, while plasma ALT and AST activities and erythrocyte membrane MDA were significantly decreased. With the increased dose of anthocyanins, the effect increased gradually, showing a dose-dependent relationship. In addition, histopathological examination showed only slight hepatosteatosis. These results indicate that anthocyanins effectively protected mice from CCl4-induced liver injury by attenuating lipid peroxidation. (3) In the cell experiment, L-02cells were treated with CCl4. After treatment, compared with the control group, ALT and AST were significantly higher in culture, which indicated that L-02liver cells had been damaged at this time, confirming successful replication of the model.Anthocyanins given prior to CC14to protect each dose group during48-h incubation can significantly attenuate the increase of ALT and AST in the cell culture supernatant caused by CC14damage, enhancing the antioxidant capacity of liver cells, scavenging free radicals, and inhibiting lipid peroxidation. This effect showed an obvious dose-response relationship (P<0.05).