| In recent years, along with the wide application of antimicrobial drugs, antimicrobial drug resistance problem has become more and more serious,these lead to drug clinical curative effect of the lower, and even invalid, infection of livestock and poultry significantly higher morbidity and mortality. While the existing problems of bacteria resistant drug control more difficult, develop resistance inhibitors gradually become the hot spot in the field of medicine. In the light of how to solve problems bacteria resistant, foreign researchers have developed a part of the resistance inhibitors, such as reserpine, cyanogen chlorobenzene hydrazone (CCCP),2-nitro phenol (DNP), although its resistance to all have certain inhibition effect, but its own toxic also. Traditional Chinese medicine is China’s unique natural resources,with a character of widely resources and effect and low side effect,in the treatment of infection, the reversal (or inhibit) tumor cells resistance etc showed particular role. This Suggests we find in Chinese traditional medicine with both good resistance to eliminate the effect is none side effects or side effects of low resistance inhibitors.The test is select clinical common100kinds of Chinese traditional medicine as the research object, screening of multiple drug-resistant Escherichia coli quinolone medicine has the discharge pump inhibitory effect of the Chinese medicine active ingredient, developed resistance to new inhibitors lay the foundation.1. The establishment of the model which have a single multidrug resistance mechanism of Escherichia coliMakke the20multiple drug-resistant E. coli which have been identification and preservation as samples, extracted of DNA and amplification of the mutations resistance-determining regions of gyrA and parC genes in PCR, and products were sequenced and analysted.By real-time quantitative fluorescence application PCR technology, detected pump gene acrA expression quantity, and application of CCCP for discharge pump inhibitors for testing, high expression and the CCCP treatment, a bacterium body ciprofloxacin lactate increased volume of the most remarkableness, established as the test with a single discharge mechanism for the escherichia coli model.Results:In20multiple drug-resistant strains Escherichia coli, gyrA and parC gene mutations not have two plants, and73-2existing discharge pump gene expression high level, so will the strains of bacteria as follow-up tests of bacteria model.2. Screening of Chinese medicine bacteria pump inhibitorsWith water extraction method and alcohol abstraction method, in view of the traditional Chinese medicine preparation every blindly two traditional Chinese medicine liquid reserves, by adopting the American clinical laboratory standards committee (NCCLS) recommended broth dilution method, determination of ciprofloxacin lactate and200kinds of Chinese traditional medicine extract on antibiotic resistant bacteria and control the bacteria ATCC25922minimum antibacterial concentration (MIC), and will Chinese herb extract and ciprofloxacin lactate joint, testing the ciprofloxacin lactate synergy.Results:Get Chinese medicine liquid reserves200species, including with drug resistance escherichia coli has bacteriostasis with142kinds, antibacterial rate is71%; Existing joint bacteriostatic effect4, respectively is licorice water decoction, ten credit ethanol-extraction fluid, asarum ethanol-extraction fluid and powder and has ethanol-extraction fluid. Resistance performance for the inhibition to escherichia coli resistance model4～64times drop MIC;124kinds of Chinese herb extract and ciprofloxacin lactate performance/for antagonist role.3. The Chinese herb extract effective on anti-deflagration strains ciprofloxacin lactate the influence of the volumeApplication of high performance liquid chromatography (HPLC) method to detect and control the bacteria resistant bacteria were to join the antibacterial concentrations before and after four Chinese herb extract, bacteria body ciprofloxacin lactate (Cip) volume of.Results:Four kinds of traditional Chinese medicine, quality control bacteria and antibiotic resistant bacteria body ciprofloxacin lactate all volume increased, added the A, Cip volume change the most significant, and3.7times.Conclusion:The antibacterial Chinese herb extract can increase escherichia coli antibiotic resistant bacteria and control the bacteria in the body of ciprofloxacin lactate volume, show good discharge of inhibition.4. The influence of Chinese herb extract on the with acrA gene expressionThe multiresistant E. coli was cultured in culture containing with Chinese herb extract and tested acrA gene expression by fluorescence Quantitative PCR technology (Real-Time Fluorescent Quantitative PCR).Results:The acrA expression was reduced after treatment, treated by A,B,C,D the E. coli discharge pump gene expression quantity was0.19,0.33,0.24, and0.16times of original respectively. Conclusion:Indicates that the Chinese traditional medicine has has apparent inhibition in the acrA gene expression quantity... |
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