Phosphorus Efficient Varieties Selection And Cloning And Sequences Analysis Of Phtl Gene From Aegilops Tauschii And Wheat

Aegilops tauschii, donor of the D genome of Triticum aestivum, not only have genetic variationmore abundant than wheat, but also have the fine features like disease resistance, drought resistance, andother stress tolerance. Through hybridization of Aegilops tauschii and wheat or indirectly to theformation of synthetic tetraploid of wheat and Aegilops tauschii, Aegilops tauschii genes can betransferredinto wheat for improving varieties and quality of weat as important genetic resources.This study through207Aegilops tauschii of the laboratory collect color reaction and material ofseedling root morphological structure change of observation and statistical, on Aegilops tauschii andwheat analysis of of low-phosphorus tolerance and to filter out some of the materials of the Pht1genecloning and sequence analysis, the main results are as follows:(1)The classification of the color reaction of rhizosphere and8low-phosphorus tolerance Aegilopstauschii material gain: Phosphorus deficiency and full40days of planting materials in mediumcontaining bromocresol purple indicator color6hours after it reaches the roots of acid secretion of themost substantial, choose medium discoloration more obvious in the rhizosphere of plants is the toleranceto low phosphorus species. Its color is divided into five levels according to the color change, through thismethod filters out8materials growth better under phosphorus deficiency conditions and color reactionmore obvious from207Aegilops tauschii materials as follows: xj006, xj35, xj96, xj101, xj112, Y57,As85, SX-11.(2)The morphological changes on resistance low phosphorus material is clear: Used improved ofHoagland nutrition liquid training test on display color reaction better and known phosphorus efficient wheat material for seedling of morphology and biomass comparison analysis. Results indicates that:deficiency phosphorus training of varieties root axis length increased, lateral roots number increased, hairincreased, root/shoot ratio(r/s) increased, but different genotypes varieties show the responsecharacteristics of significant differences. Low phosphorous tolerant varieties of r/s trend is more obvious,with low phosphorus induced changes more quickly.(3)8new Pht1gene got and the sequence analysis: Based on the reported phosphate transporter geneTaPT8of wheat, a pair of specific primers was designed,3new alleles in wheat and5new alleles inAegilops tauschii were isolated by RT-PCR from the total RNA of phosphate-staved root by28days.They have a length of1578bp, which is similar to the expected length of the coding region, by BLASTTaPT8nucleotide sequence similarity reach of95.4%~98%.Through these8amino acid sequenceanalysis of novel genes and protein structure of figure, found contain the character sequenceGGDYPLSATIxSE of the Pht1family, signal peptide sequences for the first36amino acids, has12transmembrane domains, secondary structure with MFS in the Super family domain.Proof of the cloningof genes belonging to the Pht1family members, this is the first time rough goat grass cloned out the genefamily members.