The Functional Verification Of Genetic Transformation Maize Of ZmZIP4 Gene

Zinc is an essential trace element to plants, which plays an important role in many biochemical reactions in plants. Zinc is not only a cofactor of many enzymes, but also an important structure cofactor of protein in plants, in which is not only involve body’s various metabolism, at the same time it also plays an important role in stability for biofilm and gene expression and other physiological functions. Maize is one of the crops that sensitive reactions for trace element zinc, which its biosynthesis will be in vivo blocked and the yields would be reduce remarkably in severe cases. Therefore, metal ions are essential to plants’normal development. Automatic adjustment balancing mechanism of plants can regulate the absorption and transport of metal ions to control the content of metal ions. Member of the ZIP (ZRT and IRT-like protein) gene family are widely present in animals and plants.They are capable of transporting a variety of cations, including cadmium, iron, zinc and manganese.These founding are very important for us to understand the metal deficiency or over accumulation at molecular level. The paper uses transgenic methods to transform ZmZIP4 genes into maize Hi-Ⅱspecies to identify the gene’s effect and to improve corn’s zinc absorption capability. Main studies are as follows:(1) After callus screening NX-531,NX-85, p53, BS110, JI853, Zheng 58, agriculture department of 85 could induce typeⅡorganization and can be survival once was adjusted its medium.(2) Maize endosperm-specific expression vector pr-ZmZIP4 is built on pr754 carrier, which would transfer by gene gun after its vector expressing nuclear mixing with bar gene expression nuclear. There are four resistant calluses, three of them are gene-positive callus which containing the target gene that after double PCR detecting of target gene and bar gene.(3) Maize’s over expression vector pC3301-ZmZIP4 receives seven resistant callus and shows positive by PCR assay, which build on pCAMBIA3301 carrier. ZmZIP4 gene is over express in transgenic plants that by qRT-PCR real-time quantitative PCR analysis.(4) Do a gradient low-zinc threat to pC3301-ZmZIP4 that in the process of seedling under transforming heal. To observe morphological changes of embryoid seeding with different concentrations, which illustrates ZmZIP4 plays an important role to the absorption of zinc.