Study On The Hepatoprotective Effect Of The Angelica Polysaccharide And Its Different Processed Polysaccharide Based On The Metabolomics Method

In order to explore the hepatoprotective effect of the Angelica Polysaccharide （APS） anddifferent processed Angelica polysaccharide, metabonomics method combined with thepathological and histological method and biochemical indicators testing method were used inexperiment.30ICR mice were randomly divided into the blank group, CCl₄model group,APS high-dose group （240mg/kg）, APS middle-dose group （120mg/kg） and APS low-dosegroup （60mg/kg）.The left lobe of liver tissue was taken for HE stain so as to furtherpathological and histological observe. Part of the liver tissue was taken for liver tissuehomogenates which was used to detect the MDA content and the SOD activity. Metabolomicsmethod was used to analysis of plasma and liver tissue.1. The hepatoprotective effect of different doses of the Angelica Polysaccharide andAngelica polysaccharides of different processed products liver injury induiced by CCl₄.Pathologically and histologically method, the results showed that different doses of angelicapolysaccharide all had diferent degrees of therapeutic intervention on CCl₄induced liver injury.Liver cells of Angelica polysaccharide treatment groups were observed basic normal, celledema almost disappeared and the cell cord clearly visible, which showed that CCl₄inducedliver injury had recovered. APS middle-dose group had a powerful therapeutic intervention onccl4induced liver injury. APS of different processed products also had diferent degrees oftherapeutic intervention on the liver injury induiced by CCl₄and carbon angelicapolysaccharide had a relativly powerful therapeutic intervention on ccl4induced liver injury.2. Detecting the MDA content and the SOD activity of the liver tissue. The groups of thedifferent doses of Angelica polysaccharide had varying degrees of influence on the MDAcontent and the SOD activity. The results showed that SOD activity was significantly elevated（P<0.05）and the content of MDA decreased significantly（P<0.05）in middle dose group,which had the best antioxidant capacity. The APS of different processed products treatmentgroup also had varying degrees of influence on the MDA content and the SOD activity, andthe results showed that SOD activity was significantly elevated and the content of MDAdecreased significantly in carbon angelica polysaccharide group.3. GC-MS was used to analyze the influence of three different doses of APS and Angelica polysaccharides of different processed products on metabolomics data. The PCAmethod was used to analyzing the data obtained from GC-MS metabolomics. The resultsshowed that the score plot had a clearly separation between middle doses of angelicapolysaccharide treatment group and the CCl₄model group. Comparing the content ofbiomarkers,the result showed that Butyric acid and palmitic acid content increased in plasmaand decreased in liver tissue, Malic acid, citric acid, fumaric acid, glycine and valine contenthave shown a drop and arachidonic acid and16-carbon acid had a slight increase in the bloodand liver tissue. These changes of biomarkers content were relativly abvious in the middledose group and model group. Carbon Angelica polysaccharide group compared with CCL4model group changed a lot, significant difference（P<0.05）.The experiment results also indicated that different doses of Angelica polysaccharide andall of the four different processed Angelica polysaccharides can ease CCl₄-induced liver injuryat varied degrees, it showed that biomarker content had a various change, histopathologicalobservating of liver cells came to the normal, SOD activity increased and the content of MDAdecreased. And the hepatoprotective effect of carbon Angelica Polysaccharide which had agreater change in its biomarker content was most obvious. GC-MS metabolomics was used tostudy the mechanism of CCl₄induced liver injury. CCl₄model group and the control group`sdata obtained from GC-MS metabolomics was analysed by PCA method.The score plot oftwo groups was clearly separated and it indicated that CCl₄molding was successful. VIPmethod was used to find the difference biomarkers of the two groups. The differencebiomarkers included butyric acid, palmitic acid, arachidonic acid, hexadecenoic acid, malicacid, citric acid, fumaric acid, glycine, valine. The content change of these differencebiomarkers which has important clinical and research significance was the material basis ofthe degree of liver injury. These biomarkers can be used for target detection to determine thedegree of liver injury and the effect of therapy on clinical treatment and research in the future.And it has important significance.