The Study Of Photoperiod-sensitive Genic Male Sterile Rice Fertility Alteration Sensitive Parts And Functional Analysis Of Pms3 Candidate Genes

Photoperiod-sensitive genic male sterile rice (PSGMSR) has the characteristics that its pollen fertility is regulated by photoperiod; it is completely sterile under long-day conditions but fertile under short-day conditions. PSGMSR has now been widely used in two-line hybrid rice breeding. Despite extensive studies on various aspects of the PSGMSR, the leaves and the sites where photoperiod response occurs to induce fertility change is unknown. In this study, we investigated the sites of response by examining pollen fertility of plants with differential short-day treatments of the leaves as well as different parts of the leaves, by bagging various parts of the original mutant Nongken 58S(NK58S). The results showed that the uppermost two leaves are responsible for photoperiod response for sterility induction. The uppermost unexpanded leaf is the most critical for photoperiod response, and the second leaf and its sheath played important role in enhancing the fertility response. Short-day treatments of different parts of the leaves also have different effects on fertility. Covering the upper parts of the two uppermost leaves did not affect fertility, while bagging the lower parts of the leaves resulted in fertility alteration. In addition, the treatments that increased fertility also accelerated the heading by approximately ten days, indicating that photoperiod responses of the pollen fertility and flowering may be governed by related processes.A lot of work have done to location the gene which control the fertility alteration in NK58S, finally found that when a special fragment HB614 translation into NK58S, one of the T1 generation become fertile under long-day conditions. In order to confirm the DNA fragment HB614 contain the pms3 gene or not, we use the T2 transgene plants as large population for the co-segregation test. Using Nongken 58 as donor line and Nongken 58S as receptor line. In the long day condition, we investigate the pollen and spikelet fertility, combining the CAPS and GUS makers, the result indicate that the phenotype and genotype co-segregate in the T2 population. And the DNA fragment HB614 may contain the rice photoperiod-sensitive genic male sterile gene pms3. Two express gene Gene SNP and Gene M which found in HB614 by bioinfomation predict was think as candidate gene of pms3. RT-PCR and sub-cellular localization both to done to confirm the prediction. The expetion profiles results show that the pms3 candidate genes have no photoperiodic rhythm, However, in long-day conditions, Gene M expression was higher than short-day conditions, Gene SNP expression in different sunshine conditions did not change significantly. Subcellular localization results show that, Gene M protein located in the nucleolus, while Gene SNP protein in the cytoplasm was diffuse punctate distribution, not yet clearly defined.