Study On Bifidobacterium Bifidum Microcapsules And Its Application

As the bifidobacteria had superior health properties, there were more andmore people considered it would be the most promising probiotic. Moreover,normal survival in the gastrointestinal tract depends on the strain andspecies-specifc resistance to the low pH in gastric juice and to the bile saltsfound in the small intestine. In order to solve this technical problem. Firstly, theemulsification method was adopted and the sodium alginate was used asmicroencapsulated materials in the article. The preparation technology ofmonolayer Bifidobacterium BB01and BB28microcapsules were optimized bysingle factor and response surface method. On the above basis, the preparationtechnology of double and triple Bifidobacterium BB01and BB28microcapsuleswere studied by using chitosan and palm oil respectly, and the process conditionof monolayer and double Bifidobacterium BB01and BB28microcapsules wasoptimized. Finally, the characteristics and application in the fruit orange,nutrition express and the pure milk of monolayer, double and triplemicrocapsules were studied. The main results were as follows:1)The optimal condition for preparing monolayer Bifidobacterium BB01was with proportion of Bifidobacterium and sodium alginate was1:3, sodiumerythorbate content of0.12%, the inulin content of6%, the immobilization time15min, emulsification time was12.5min, CaCl2concentration of0.8%, theproportion of oil and water was4.8:1, concentration of sodium alginate was1.6%and tween content1%. Under such process condition, the encapsulationyield(EY) and viable counts of monolayer Bifidobacterium BB01microencapsulation was81.81%and2.67×109cfu/g respectly; The optimalcondition for preparing monolayer Bifidobacterium BB28was with proportionof Bifidobacterium and sodium alginate was1:11.5, sodium ascorbate content0.0675%, oligofructose content6%, immobilized time15min, theemulsification time l5min, CaCl2concentration1%, the proportion of oil andwater was3.2:1, sodium alginate concentration is1.9%, tween content0.5%. Under such process condition, the encapsulation yield(EY) and viable counts ofmonolayer Bifidobacterium BB28microencapsulation was91.97%and2.14×109cfu/g respectly;2)The encapsulation yield(EY) of double Bifidobacterium BB01and BB28microencapsulation were influenced by the pH of the chitosan solution, thechitosan concentration and microencapsulated time. The level of main factorswere decided when the maximum response area was found by the design ofsteepest ascent. The optimal condition for preparing double BifidobacteriumBB01and BB28microcapsules was optimized by the design of Box-Behnken.The results were as follows: The optimal condition for preparing doubleBifidobacterium BB01and BB28microencapsulation were all with the pH ofthe chitosan solution was4.5, and the chitosan concentration of1%andmicroencapsulated time15min. Under such process condition, theencapsulation yield(EY) and viable counts of double Bifidobacterium BB01microencapsulation was85.77%and2.16×109cfu/g. The encapsulationyield(EY) and viable counts of double Bifidobacterium BB28microencapsulation was87.89%and2.12×109cfu/g.3)Trilayer microcapsules was prepared by using palm oil as the wallmaterial after preparing double microcapsules. The encapsulation yield(EY) oftrilayer Bifidobacterium BB01and BB28microencapsulation was almost100%.The viable counts of1g trilayer Bifidobacterium BB01microcapsules is2.16×109cfu/g; The viable counts of1g trilayer Bifidobacterium BB28microcapsules is1.97×109cfu/g.4)Compare monolayer microcapsules, double microcapsules and to freebifidobacterium, the properties of monolayer microcapsules, doublemicrocapsules and trilayer microcapsules was better than free bifidobacterium,especially in the resistance to gastric acid, bile resistant and in the foodapplications. Bifidobacterium who was microencapsulated have a distinctadvantage over free bifidobacterium. The experimental result of cidproof andcholateproof in2hours showed that the viable counts of free bifidobacteriumBB01and BB28soon decreased to0after2hours. The viable counts ofmonolayer microcapsules, double microcapsules and trilayer microcapsulesdecreased by nine magnitudes, four magnitude and one magnitude respectly. The acid resistance and bile tolerance of bifidobacterium BB28is better thanbifidobacterium BB01. The enteric test showed that monolayer microcapsules,double microcapsules and trilayer microcapsules could release completely in40min.5)The application of free bifidobacterium, monolayer microcapsules, doublemicrocapsules and trilayer microcapsules in fruit orange and nutrition Expressshowed that the rate of decrease of viable counts of free bifidobacterium,monolayer microcapsules, double microcapsules and trilayer microcapsulesdecreased in sequence under4℃. The optium storage time of freebifidobacterium, monolayer microcapsules, double microcapsules and trilayermicrocapsules was21days,21days,28days and more than35days. The viablecounts were maintained at1×106cfu/g or more, and the acidity and pH changesare not very significant under4℃.The rate of decrease of viable counts of freebifidobacterium, monolayer microcapsules, double microcapsules and trilayermicrocapsules aslo decreased in sequence under the conditions of ambienttemperature storage. The optium storage time of free bifidobacterium,monolayer microcapsules, double microcapsules and trilayer microcapsules was7days,14days,21days and more than28days. The viable counts weremaintained at1×106cfu/g or more, and the acidity and pH changes are not verysignificant under the conditions of ambient temperature storage. The stability ofBB28is better than BB01.The viable counts of free bifidobacterium, monolayermicrocapsules, double microcapsules and trilayer microcapsules all sawincreases in pure milk under4℃and the conditions of ambient temperaturestorage. The acidity and pH changes are not very significant.In this study, the problem of Bifidobacterium was sensitive to disadvantageenvironment was resolved by using the microcapsule technique.The survivalof Bifidobacterium was enhanced. The study has laid the theoretical basis andpractice foundation for the application of bifidobacteria microcapsules in foodand the development of a new type of probioticthe microcapsules products.