Design And Synthesis Of Thiazoles Plant Activators And PAL Fluorescent Probe

Plant activators do not have bactericidal and bacteriostatic effect or only low inhibitory and bactericidal activity in vitro, but can induce the plant’s immunity to pathogen in vivo. Therefore plant activator can not generate selected pressure and drug resistance, and is friendly to the environment. In this thesis, we designed two series of compounds, which include28thiatriazole derivatives and4thiadiazole derivatives. Among these compounds, N-(4-(trifluoromethyl)phenyl)-1,2,3,4-thiatriazol-5-amine can induce plant’s resistance to six kinds of pathogen which include Mycosphaerella melonis, Corynespora cassiicola, Pseudomonas syringae, Phytophthora infestans, Thanatephorus cucumeris, Botrytis cinerea and Erwinia amylovora, and the activity in vivo is much better than in vitro, which behaves like the common plant activators.It has been found that the level of some signaling molecules and proteins would be elevated in the process of resistance response elicited by plant activators, and PAL is one of these significant enzymes, which was taken for a maker for the induced resistance. Base on the reaction catalyzed by PAL, we designed and synthesized an OFF-ON fluorescent probe for PAL. Probe2can lead15-fold fluorescence enhancing after reaction with PAL for1hour and can ratiometrically detected PAL at495nm.