Synthesis And Fluorescence Properties Of Novel Fluorescent Probes For DNA Sequencing

Fluorescent probes have been widely used in biochemistry, analytical chemistry, chemical engineering and medicine engineering in recent years. Chemists and biologists continue to design and synthesize various new fluorescent probes to meet the different needs of fluorescent tests. The synthesis and application reseach of the fluorescent probe dyes have become the hot point in interdisciplinary of life scince and chemistry.In this thesis, a series of novel fluorescent probes for DNA sequencing were designed and synthesized. Summarized as follows:1. Four fluorescein dyes were synthesized. Structures of the target compounds were determined via IR, MS,1H NMR. After examining their photophysical properties, we found that different number of chlorine substitutions which are at the different positions have different effects on the fluoresceins.2.6-carboxylfluorescein,2',7'-dichloro-6-carboxylfluorescein,4',5'- dichloro-6-carboxyl-fluorescein and 4',5'2',7'-tetrachloro-6-carboxylfluorescein isomers were synthesized and separated sucessfully. Structures of the target compounds were determined via IR, MS,1H NMR.3. Four novel fluorescent probes with 6-aminohexanol linker for DNAsequencing were synthesized. Structures of the target compounds were determined via IR, MS,1H NMR. In order to investigate the fluorescent properties, we convert the fluorescent probes to the quinoid form with the removal of protective groups.4. After examining the photophysical properties of the fluorescent probes, we found that different number of chlorine substitutions which are at the different positions has different effects on the fluoresceins. In the end, we investigated the effects of the chain link on the fluoresceins.