The Preparation Of Novel Molecularly Imprinted Polymer And Application In Drug And Food Analysis

In this thesis, four kinds of novel molecularly imprinted polymers were prepared basedon the precipitation polymerization, surface imprinting method and novel nano preparationtechnology. The preparation conditions were optimized, the polymers were characterized andadsorption was studied. And then, the molecularly imprinted polymers were used in flowinjection chemiluminescence analysis. Four kinds of molecularly imprinted polymers-chemiluminescence （MIP-CL） sensors were established and used into the drug and foodsamples detected analysis. There were five parts in this thesis.The development and present research status of the molecular imprinting technology andmolecularly imprinted polymers was reviewed in the first part. The concept, research process,classification, preparation methods and applications of the molecular imprinting techniquewere introduced in this part. And then the novel molecularly imprinted technology wasemphatically introduced. Besides, the preparation method and research process of themolecularly imprinted polymers flow injection chemiluminescence （MIP-CL） sensor weredescribed.The magnetic shell chrysoidine molecularly imprinted polymers were prepared by surfaceimprinting technology. At first, Fe₃O₄nanoparticles were prepared by coprecipitation, then theFe₃O₄@SiO₂magnetic nanoparticles were prepared by coated SiO₂layer, the silanization ofFe₃O₄@SiO₂was reacted by3-（trimethoxysilyl）propyl methacrylate （MPS）. And then, themagnetic shell chrysoidine molecularly imprinting polymers were prepared based on silanizedFe₃O₄@SiO₂magnetic nanoparticles as carriers. The preparation conditions were optimized,the polymers were characterized and adsorption was studied. And the imprinted polymersexhibited excellent magnetic response and adsorptive capacity. The chrysoidine MIP-CLsensor was prepared combined with chemiluminescence, and the conditions were optimized.The linear range of the sensor was2.0×106¹.0×104mol/L, and the detection limit was6.2×10^-7mol/L. At last, the chrysoidine samples were detected efficiently by MIP-CL sensor.The magnetic shell sulfadiazine MIPs were prepared by the same surface imprintingtechnology. The preparation conditions were optimized, the polymers were characterized andadsorption was studied. The magnetic MIP exhibited strong magnetic response and excellentadsorptive capacity. A novel MIP-CL sensor was prepared combined with flow injectionchemiluminescence, and chemiluminescence conditions were optimized. The working curveof the sensor was ICL=114.0+11.0×107c （R2=0.9943）, the linear range of the sensor was4.0×10^-71.0×10^-4mol/L, and the detection limit was1.5×10^-7mol/L. At last, the sulfadiazinesamples were detected with high sensitivity and high selectivity by MIP-CL sensor.The core-shell dapsone MIPs were prepared by surface graft technology. Firstly, SiO₂nanoparticles were obtained by hydrolysis of tetraethoxysilane. Secondly, SiO₂nanoparticleswere silanized by （3-aminopropyl）triethoxysilane （APTES）. And then, the core-shell dapsoneMIPs were prepared based on silanized SiO₂nanoparticles as carriers. The preparationconditions were optimized, the polymers were characterized and adsorption was studied. And the dapsone MIP-CL sensor was prepared, the chemiluminescence conditions were optimized.The linear range of the sensor was1.0×10`6¹.0×10⁴mol/L, and the detection limit was5.3×10^-7mol/L. At last, the dapsone samples were detected with high sensitivity and highselectivity by MIP-CL sensor.The phenylalanine MIPs were prepared by precipitation polymerization method. In theexperiment, ethanol, acrylamide, ethylene glycol dimethacrylate,2,2-azobisisobutyronitrilewere choosed as the solvent, functional monomer, crosslinking agent and initiator, respectively.The preparation conditions were optimized, the polymers were characterized and adsorptionwas studied. The phenylalanine MIP-CL sensor was prepared combined chemiluminescence,and luminol-H2O2-NaOH was selected as chemiluminescence system. The linear range of thesensor was5.0×10^-79.0×10^-5g/mL, and the detection limit was1.1×10^-7g/mL. At last, thephenylalanine samples were detected with high sensitivity and high selectivity by MIP-CLsensor.