Extraction Of Volatile Oil Of Bidens Pilosa L And Study Of Inclusion Process

Bidens volatile oils have many pharmacological effects. Case of light and heat unstable with strong volatility due to volatile oil, water-soluble because of poor seriously affect its production. The domestic and foreign literature has not been reported Bidens volatile oil extraction and inclusion process.To discuss the best extraction technology of the volatile oil of Bidens bipinnata L, and to compare the value of the extraction of each part of Bidens bipinnata L. Adopt hydro distillation, According to the determination of volatile oil in Appendix XD of the first department of the China Pharmacopoeia edition2010, take the yield as the index using orthogonal experiment to optimize the extraction technology of the volatile oil of Bidens bipinnata L.。The best extraction technology of the volatile oil of bidens bipinnata L.by hydrodistillation was as follow:crush degree of herb of65mesh, extraction time of5h and the proportion of material to solvent of1:7。Using steam distillation essential oil, respectively saturated aqueous solution method, liquid-liquid inclusion method, gas-liquid preparation of Bidens bipinnata adduction (3-cyclodextrin inclusion volatile oil to inclusion yield, essential oil inclusion rate composite score index, chosing the best method of inclusion. Saturated aqueous solution, liquid-liquid inclusion method, gas-liquid inclusion comprehensive scores were38.46%,66.17%,53.60%.Liquid-liquid inclusion comprehensive score was the highest, and operation is simple and convenient. It’s the preparation of essential oil of Bidens bipinnata (3-cyclodextrin inclusion complex the ideal method,。TO identify β-cyclodextrin inclusion complex of Bidens bipinnata essential oil Microscopy, UV spectrophotometry were the methods of identification. The results confirmed that a new phase was formed, the microscope and UV spectra confirmed the Bidens bipinnata essential oil after the encapsulation of β-cyclodextrin.