Study On Processing And Syability Of Artemisia Argyi Volatile Oil-β-cyclodextrin Inclusion Compound

Artemisia vulgaris was the dried leaves of Artemisia argyi Levl.Et Vant, which was bitter, acrid, warm and went through the spleen, liver and kidney. It have many functions, such as warm blood, dispel cold, release pain, cure cough and asthma, Stop-leak tocolysis, dampness itching etc. The main medicinal components was volatile oil, having smooth over asthma, antitussive, expectorant and anti-bacterial inflammatory effects. However, volatile oil has a strong volatility and pungent, unstable in case of light and heat, poor solubility, that seriously affected its application. Volatile oil will be more stability and availability as well as improving the application, by using of cyclodextrin technology. The purpose of this project is to extract oil from the leaves, by using three different techniques to get a better optimized technology. Then compare the stability of the complex extracted. The results of the studies are as follows:(1) Volatile oilβ-cyclodextrin complex:The utilization ratio of the volatile oil from Artemisia argyi, the content of volatile oil in inelusion compound and the recovery ratio of inclusion counpound were as criteria. The three processes were studied by orthogonal experimental design using orthogonal form (L9(43)) and compare the methed of trituration,the methoed of saturation water solution, supersound method.By three methods best preparation conditions inclusion 3 batches of samples, determination of ultrasonic, saturated aqueous solution method and grinding method average inclusion rate was 50% and 93% and 55%. From the results, the method of saturation water solution was confirmed as the better technology for preparing the aetherolea P-CD clathrate, with higher inclusion rate and more suitable for large-scale production.(2.) Volatile oilβ-cyclodextrin phase identification:Microscopy, thin layer chromatography, UV spectrophotometry were the methods of identification. The results confirmed that a new phase was formed inβ-essential oil Cyclodextrin encapsulation through the microscope and UV spectrum, after that inclusion complexes have been formed; volatile oil in the inclusion TLC before and after are consistent with, because that there are no spots in same condition. It showed that volatile oil has been with theβ-CD to form a stable inclusion complex, and in the nature of inclusion did not change before and after. The UV spectrum and Microscopy can give the formation of inclusion complexes(3) Volatile oilβ-cyclodextrin complex stability:The stability of inclusion complexes were determined by anti-photolysis experiments, thermal stability test, wet test. The result shows that the content of volatilize oil in inclusion compound did not change. In the mixtrue, however, the content of volatilize oil decreased. It showed that volatile oil in inclusion compound was much more stable under light,heat and humidity. In the volatility test, loss on drying of the compound was smaller than that of the mixture, indicating that inclusion compound could lower volatilization of volatile oil. Therefore, we can infer that when the volatile oil andβ-CD inclusion complex can significantly improve the stability and reduce volatile.In summary, essential oilβ-CD inclusion complex prepared by saturated aqueous solution was simple, low cost and less demanding on equipment, and good stability, with a certain application value.