Research On The Extraction And Functional Properties Of Soluble Glycoprotein In Egg Albumen Powder

With fresh egg white powder as raw material,this study adopts the water solubleglycoprotein extraction to extract crude products,use of glycoprotein coarse Sevage reagentmethod of removing protein purification processing, product reuse Alcalase modificationenzyme solution for the final will be egg white powder, soluble glycoprotein and its effecton the enzyme solution objects outside the S180sarcoma cells in mice, the tumor growthinhibitory activity in vitro was observed. Specific research conclusion is as follows:1,The study of soluble glycoprotein extraction process. In protein content andsolubility as the measure, the heating time, heating temperature, slurry than3on the basisof single factor experiment and research, through the Box-Behnken response surfacemethod to optimize extraction process, the best extraction process parameters for100mmNaCl concentration, temperature of57.24℃, and solid-liquid ratio1:3.16,44.17minleaching, in the process parameters under the condition of egg white powder solubility andsoluble protein content reached maximum at the same time, the solubility of95.90%,soluble protein content of73.72%. Repeat test three times, under the best conditions to getactual solubility by an average of94.88%, protein content is72.94%.2, Protein purification technology of soluble glycoprotein were studied. For proteinand polysaccharide content of soluble glycoprotein measure, inspect sevage reagentchloroform and n-butanol in protein ratio, proportion of sevage reagent is added, threefactors of time for protein purification effect, on the basis of single factor experiments,through L9(3~4) orthogonal test optimization of protein purification process, get thefollowing conclusion: in accordance with the proportion of1:2add3:1in chloroform andn-butanol sevage reagents,30min at room temperature to take off the protein, this kind ofsituation, the soluble protein content in glycoprotein, lowest available in soluble proteincontent was8.02%in glycoprotein, polysaccharide content is4.63%. 3, The soluble glycoprotein and digestion object to study the antitumor activity.Different concentrations of egg white powder soluble glycoprotein have certain inhibitioneffect in vitro to S180sarcoma cells.