| In this paper the ethylcellulose which viscosity specifications is 90～110 mPa · s(EC 100)was added as a fat texture agent to the shortening samples,we investigated its characteristics as a controlled release formulation for fat-soluble vitamins(vitamin A,vitamin E and β-carotene)by means of an in vitro digestion,and we clarified the mechanism of sustained release.By the rats fed experiment,we studied the effect of ethylcellulose on animal nutrition physiological functions including cardiovascular disease risk factors.In the study of the characteristics of EC 100 as a controlled release formulation for fat-soluble vitamins,we knew that the sustained release action of EC 100 organic gel showed a increasing trend with the increasing added amount of EC 100.While the mass fractions of EC 100 ranged from 0%to 6%,the cumulative release rate of vitamin A,vitamin E and p-carotene decreased by 16.9%,53.41%,17.15%respectively when the shortening samples had been digested for 2 hours.By comparing the release rate of three fat-soluble vitamins at the same point of digest time we knew that the release rate of vitamin E was about three times much than other two vitamins,and release rate of β-carotene and vitamin A release rate were closer,both in the EC 100 organic gel had a similar release characteristics.With being fitted by Ritger-Peppas equation,the dissolution parameters appeared within the scope of 0.45～0.89,which implied the release rate of three kinds of fat-soluble vitamins were controlled doubly by compounds diffusion and matrix erosion.The measurement results of the diffusion coefficient showed that the three kinds of fat-soluble vitamins concentration gradient were the driving force of diffusion,and the vitamins were spreading from the high concentration to low concentration,the diffusion coefficient of three fat-soluble vitamins reduced with the increasing concenrtation of EC100,and the range of diffusion coefficient of VA,β-carotene and VE were 21.4×10-5～3.37×10-5mm2/s、19.1×10-5～2.25×10-5mm2/s and 34.4×10-5～13.37×10-5mm2/s respectively.The diffusion coefficient of VE was maximum,the diffusion coefficient of VA and β-carotene were closer to no significant differences.Erosion constant measurement results showed that the skeleton dissolution was one of the mechanisms for sustained-release of EC 100 oil products.Decreases in erosion constant were determined with increasing concentration of EC 100,while the added amount of EC 100 increased from 0%to 6%,the erosion constant of samples stored for one month reduced by 0.15h-1 when campared with samples stored for three month.The erosion constant of the organic gel samples decreased with the prolongation of storage time,organic gel samples containing 3%EC 100 storaged for three months showed a erosion constant which was 0.08h-1 smaller than that storaged for one month.The erosion constant of the organic gel samples increased with the rising of storage temperature,compared with samples storaged at 37℃,the erosion constant of samples storaged at 4℃ reduced by 0.06h-1.Samples adding different emulsifiers showed different erosion constants:Molecular distilled monoglycerides<Trimer glycerol monoglycerides<Span 60<Mono glyceryl stearate.Rats nutritional physiological data showed that the low density lipoprotein levels,total cholesterol and atherogenic hardening index of rats that fed the EC 100 shortening samples showed an increasing trend while compared with the blank control group,these suggested that EC 100 had some properties of reducing blood fat and cholesterol.The SOD value in vivo of rats which fed a sample containing 9%EC 100 was 31.06 U/mL higher than the blank control group,this indicated that EC 100 had some antioxidant properties.Other nutritional physiology data suggest that the shortening products with ethyl cellulose organic gel did not have adverse effects on animal organisms. |
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