The Synthesis And Properties Of Nitric Oxide Fluorescence Probes

NO is the smallest and most simple biological molecular found in vivo，it playan important role in the life course,it is very easy to fat-soluble,and cross the cellmembrance,studies have shown that NO is closely related to various disease ofcardiovascular system, nervous system and respiratory system it was namedmolecular of in the year in1992by Scienc.NO is produced as needed by the enzymenitric oxide synthase（NOS），due to the instability of NO,the analysis of NO isdifficult，the method to analysis NO has become a hot topic in the biological analysisfield，while fluorescence analysis with the advantage of fast、specificty andsensitive has caused a widespread concern by the scientist.The paper individed intofour chapters on nitric oxide fluorescent probes including research progress、designprinciples、synthesis and properties of the study.In the first chapter, the paper introduces the role of NO in human body，overviewes the research progress, design principles、synthesis of nitric oxidefluorescence probeIn the second chapter，we synthesized a new fluorescent probe substituted bytert-butyl in7position from tert-butyl phenol, phthalic anhydride, N,N-diethylamino phenol, o-phenylenediamine and characterized by IR, NMR，wehave found in fluorescence scanning experiments that when exciting at λex=489nm，the fluorescent probe exhibits a maximum emission wavelength at λex=521nm at25℃in pH=7.4.,0.1mol/L PBS. Fluorescence properties experiment shows that itstable to temperature and pH and is specificity for NO to NO₂^-、H₂O₂、O₂-,but wealso found that the probe has a long reaction time to NO and a short maximumexcitation wavelength，which still need to be improve.In the third chapter，we synthesized another new fluorescent probe substitutedby chlorine in6position from inter-chlorophenol, phthalic anhydride, N,N-diethylamino phenol, o-phenylenediamine and characterized by IR, NMR，wehave found in fluorescence scanning experiments that when exciting at λex=525nm，the fluorescent probe exhibits a maximum emission wavelength at λex=575nm. at 25℃in pH=7.4.,0.1mol/L PBS. Fluorescence properties experiment shows that itstable to temperature and pH and is specificity for NO to NO₂^-、H₂O₂、O₂-,and has along maximum excitation and emission wavelength but we still found that the probehas a long reaction time to NO which still need to be improve.In the fourth chapter,we summarize the experimental results and conclusions...